• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

着丝粒染色体在出芽酵母中的行为。

Behavior of dicentric chromosomes in budding yeast.

机构信息

Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.

出版信息

PLoS Genet. 2021 Mar 18;17(3):e1009442. doi: 10.1371/journal.pgen.1009442. eCollection 2021 Mar.

DOI:10.1371/journal.pgen.1009442
PMID:33735169
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8009378/
Abstract

DNA double-strand breaks arise in vivo when a dicentric chromosome (two centromeres on one chromosome) goes through mitosis with the two centromeres attached to opposite spindle pole bodies. Repair of the DSBs generates phenotypic diversity due to the range of monocentric derivative chromosomes that arise. To explore whether DSBs may be differentially repaired as a function of their spatial position in the chromosome, we have examined the structure of monocentric derivative chromosomes from cells containing a suite of dicentric chromosomes in which the distance between the two centromeres ranges from 6.5 kb to 57.7 kb. Two major classes of repair products, homology-based (homologous recombination (HR) and single-strand annealing (SSA)) and end-joining (non-homologous (NHEJ) and micro-homology mediated (MMEJ)) were identified. The distribution of repair products varies as a function of distance between the two centromeres. Genetic dependencies on double strand break repair (Rad52), DNA ligase (Lif1), and S phase checkpoint (Mrc1) are indicative of distinct repair pathway choices for DNA breaks in the pericentromeric chromatin versus the arms.

摘要

体内的 DNA 双链断裂是在双着丝粒染色体（一条染色体上有两个着丝粒）与附着在相反纺锤体极体上的两个着丝粒一起经历有丝分裂时产生的。由于产生的单着丝粒衍生染色体的范围，DSB 的修复会产生表型多样性。为了探究 DSB 是否可以根据其在染色体中的空间位置进行差异修复，我们检查了来自含有一系列双着丝粒染色体的细胞中单着丝粒衍生染色体的结构，其中两个着丝粒之间的距离从 6.5kb 到 57.7kb 不等。确定了两种主要的修复产物类别，基于同源性的（同源重组 (HR) 和单链退火 (SSA)）和末端连接（非同源 (NHEJ) 和微同源介导 (MMEJ)）。修复产物的分布随两个着丝粒之间的距离而变化。对双链断裂修复 (Rad52)、DNA 连接酶 (Lif1) 和 S 期检查点 (Mrc1) 的遗传依赖性表明，着丝粒周围染色质中的 DNA 断裂与臂部的修复途径选择不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8baf/8009378/d88c918e5a44/pgen.1009442.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8baf/8009378/88f24e98874c/pgen.1009442.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8baf/8009378/073bb564bcf2/pgen.1009442.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8baf/8009378/2b3f149ec893/pgen.1009442.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8baf/8009378/f82e091aa72a/pgen.1009442.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8baf/8009378/458060c06683/pgen.1009442.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8baf/8009378/d88c918e5a44/pgen.1009442.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8baf/8009378/88f24e98874c/pgen.1009442.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8baf/8009378/073bb564bcf2/pgen.1009442.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8baf/8009378/2b3f149ec893/pgen.1009442.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8baf/8009378/f82e091aa72a/pgen.1009442.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8baf/8009378/458060c06683/pgen.1009442.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8baf/8009378/d88c918e5a44/pgen.1009442.g006.jpg

相似文献

1
Behavior of dicentric chromosomes in budding yeast.着丝粒染色体在出芽酵母中的行为。
PLoS Genet. 2021 Mar 18;17(3):e1009442. doi: 10.1371/journal.pgen.1009442. eCollection 2021 Mar.
2
Mapping of meiotic single-stranded DNA reveals double-stranded-break hotspots near centromeres and telomeres.减数分裂单链DNA的图谱揭示了着丝粒和端粒附近的双链断裂热点。
Curr Biol. 2007 Dec 4;17(23):2003-12. doi: 10.1016/j.cub.2007.10.066.
3
A chromosome breakage assay to monitor mitotic forces in budding yeast.一种用于监测芽殖酵母有丝分裂力的染色体断裂试验。
J Cell Sci. 1994 Apr;107 ( Pt 4):891-902. doi: 10.1242/jcs.107.4.891.
4
Nuclear oscillations and nuclear filament formation accompany single-strand annealing repair of a dicentric chromosome in Saccharomyces cerevisiae.核振荡和核细丝形成伴随着酿酒酵母中双着丝粒染色体的单链退火修复。
J Cell Sci. 2003 Feb 1;116(Pt 3):561-9. doi: 10.1242/jcs.00251.
5
Position effects influencing intrachromosomal repair of a double-strand break in budding yeast.影响芽殖酵母中双链断裂的染色体内修复的位置效应
PLoS One. 2017 Jul 11;12(7):e0180994. doi: 10.1371/journal.pone.0180994. eCollection 2017.
6
Cytokinesis breaks dicentric chromosomes preferentially at pericentromeric regions and telomere fusions.有丝分裂优先在着丝粒区域和端粒融合处打断双着丝粒染色体。
Genes Dev. 2015 Feb 1;29(3):322-36. doi: 10.1101/gad.254664.114.
7
Dicentric chromosomes are resolved through breakage and repair at their centromeres.双着丝粒染色体通过在着丝粒处发生断裂和修复而得以解决。
Chromosoma. 2024 Apr;133(2):117-134. doi: 10.1007/s00412-023-00814-6. Epub 2024 Jan 2.
8
RAD52-mediated repair of DNA double-stranded breaks at inactive centromeres leads to subsequent apoptotic cell death.RAD52 介导的非活性着丝粒处 DNA 双链断裂的修复导致随后的细胞凋亡。
Nucleic Acids Res. 2024 Nov 27;52(21):12961-12975. doi: 10.1093/nar/gkae852.
9
The meiotic-specific Mek1 kinase in budding yeast regulates interhomolog recombination and coordinates meiotic progression with double-strand break repair.芽殖酵母减数分裂特异性 Mek1 激酶调节同源重组并协调减数分裂进程与双链断裂修复。
Curr Genet. 2019 Jun;65(3):631-641. doi: 10.1007/s00294-019-00937-3. Epub 2019 Jan 22.
10
The role of nonhomologous DNA end joining, conservative homologous recombination, and single-strand annealing in the cell cycle-dependent repair of DNA double-strand breaks induced by H(2)O(2) in mammalian cells.非同源DNA末端连接、保守同源重组和单链退火在哺乳动物细胞中由H₂O₂诱导的DNA双链断裂的细胞周期依赖性修复中的作用。
Radiat Res. 2008 Dec;170(6):784-93. doi: 10.1667/RR1375.1.

引用本文的文献

1
Centromeres are stress-induced fragile sites.着丝粒是应激诱导的脆弱位点。
Curr Biol. 2025 Mar 24;35(6):1197-1210.e4. doi: 10.1016/j.cub.2025.01.055. Epub 2025 Feb 18.
2
Dicentric chromosomes are resolved through breakage and repair at their centromeres.双着丝粒染色体通过在着丝粒处发生断裂和修复而得以解决。
Chromosoma. 2024 Apr;133(2):117-134. doi: 10.1007/s00412-023-00814-6. Epub 2024 Jan 2.
3
Context-dependent neocentromere activity in synthetic yeast chromosome .合成酵母染色体中依赖于上下文的新着丝粒活性

本文引用的文献

1
DNA replication machinery prevents Rad52-dependent single-strand annealing that leads to gross chromosomal rearrangements at centromeres.DNA 复制机制可防止 Rad52 依赖性单链退火，从而避免在着丝粒处发生大规模染色体重排。
Commun Biol. 2020 Apr 30;3(1):202. doi: 10.1038/s42003-020-0934-0.
2
Common Features of the Pericentromere and Nucleolus.着丝粒和核仁的共同特征。
Genes (Basel). 2019 Dec 10;10(12):1029. doi: 10.3390/genes10121029.
3
Deletions associated with stabilization of the Top1 cleavage complex in yeast are products of the nonhomologous end-joining pathway.
Cell Genom. 2023 Nov 9;3(11):100437. doi: 10.1016/j.xgen.2023.100437. eCollection 2023 Nov 8.
4
Splitting the yeast centromere by recombination.通过重组拆分酵母着丝粒。
Nucleic Acids Res. 2024 Jan 25;52(2):690-707. doi: 10.1093/nar/gkad1110.
5
Humanization reveals pervasive incompatibility of yeast and human kinetochore components.人源化揭示了酵母和人类动粒组分普遍存在的不兼容性。
G3 (Bethesda). 2023 Dec 29;14(1). doi: 10.1093/g3journal/jkad260.
6
Double-strand breaks induce inverted duplication chromosome rearrangements by a DNA polymerase δ-dependent mechanism.双链断裂通过依赖 DNA 聚合酶 δ 的机制诱导倒位重复染色体重排。
Nat Commun. 2023 Nov 2;14(1):7020. doi: 10.1038/s41467-023-42640-5.
7
Flexible Attachment and Detachment of Centromeres and Telomeres to and from Chromosomes.着丝粒和端粒与染色体的灵活连接和分离。
Biomolecules. 2023 Jun 20;13(6):1016. doi: 10.3390/biom13061016.
8
Mechanisms of DNA Mobilization and Sequestration.DNA 迁移和隔离的机制。
Genes (Basel). 2022 Feb 16;13(2):352. doi: 10.3390/genes13020352.
9
Employing CRISPR/Cas Technology for the Improvement of Potato and Other Tuber Crops.利用CRISPR/Cas技术改良马铃薯及其他块茎作物。
Front Plant Sci. 2021 Oct 26;12:747476. doi: 10.3389/fpls.2021.747476. eCollection 2021.
与酵母中 Top1 切割复合物稳定相关的缺失是同源末端连接途径的产物。
Proc Natl Acad Sci U S A. 2019 Nov 5;116(45):22683-22691. doi: 10.1073/pnas.1914081116. Epub 2019 Oct 21.
4
The regulation of chromosome segregation via centromere loops.通过着丝粒环来调节染色体分离。
Crit Rev Biochem Mol Biol. 2019 Aug;54(4):352-370. doi: 10.1080/10409238.2019.1670130. Epub 2019 Oct 1.
5
Meiotic DNA Repair in the Nucleolus Employs a Nonhomologous End-Joining Mechanism.有丝分裂 DNA 修复在核仁中采用非同源末端连接机制。
Plant Cell. 2019 Sep;31(9):2259-2275. doi: 10.1105/tpc.19.00367. Epub 2019 Jul 2.
6
A mitosis-specific and R loop-driven ATR pathway promotes faithful chromosome segregation.一种有丝分裂特异性且由R环驱动的ATR途径促进准确的染色体分离。
Science. 2018 Jan 5;359(6371):108-114. doi: 10.1126/science.aan6490. Epub 2017 Nov 23.
7
The non-homologous end-joining factor Nej1 inhibits resection mediated by Dna2-Sgs1 nuclease-helicase at DNA double strand breaks.非同源末端连接因子Nej1在DNA双链断裂处抑制由Dna2-Sgs1核酸酶-解旋酶介导的切除反应。
J Biol Chem. 2017 Sep 1;292(35):14576-14586. doi: 10.1074/jbc.M117.796011. Epub 2017 Jul 5.
8
Nucleolar reorganization in response to rDNA damage.响应核糖体DNA损伤的核仁重组
Curr Opin Cell Biol. 2017 Jun;46:81-86. doi: 10.1016/j.ceb.2017.03.004. Epub 2017 Apr 18.
9
Temporal and Spatial Uncoupling of DNA Double Strand Break Repair Pathways within Mammalian Heterochromatin.哺乳动物异染色质中 DNA 双链断裂修复途径的时空分离。
Mol Cell. 2016 Jul 21;63(2):293-305. doi: 10.1016/j.molcel.2016.06.002. Epub 2016 Jul 7.
10
Centromeric DNA replication reconstitution reveals DNA loops and ATR checkpoint suppression.着丝粒DNA复制重建揭示了DNA环和ATR检查点抑制。
Nat Cell Biol. 2016 Jun;18(6):684-91. doi: 10.1038/ncb3344. Epub 2016 Apr 25.