Tussipkan Dilnur, Manabayeva Shuga A
Plant Genetic Engineering Laboratory, National Center for Biotechnology, Nur-Sultan, Kazakhstan.
Front Plant Sci. 2021 Oct 26;12:747476. doi: 10.3389/fpls.2021.747476. eCollection 2021.
New breeding technologies have not only revolutionized biological science, but have also been employed to generate transgene-free products. Genome editing is a powerful technology that has been used to modify genomes of several important crops. This review describes the basic mechanisms, advantages and disadvantages of genome editing systems, such as ZFNs, TALENs, and CRISPR/Cas. Secondly, we summarize in detail all studies of the CRISPR/Cas system applied to potato and other tuber crops, such as sweet potato, cassava, yam, and carrot. Genes associated with self-incompatibility, abiotic-biotic resistance, nutrient-antinutrient content, and post-harvest factors targeted utilizing the CRISPR/Cas system are analyzed in this review. We hope that this review provides fundamental information that will be useful for future breeding of tuber crops to develop novel cultivars.
新的育种技术不仅彻底改变了生物科学，还被用于生产无转基因产品。基因组编辑是一项强大的技术，已被用于修饰多种重要作物的基因组。本综述描述了基因组编辑系统（如锌指核酸酶、转录激活因子样效应物核酸酶和CRISPR/Cas）的基本机制、优缺点。其次，我们详细总结了CRISPR/Cas系统应用于马铃薯和其他块茎作物（如甘薯、木薯、山药和胡萝卜）的所有研究。本综述分析了利用CRISPR/Cas系统靶向的与自交不亲和、非生物-生物抗性、营养-抗营养成分以及采后因素相关的基因。我们希望本综述能提供基础信息，有助于未来块茎作物培育新的品种。
