通过电子晶体学对破伤风毒素进行三维结构分析。

Three-dimensional structural analysis of tetanus toxin by electron crystallography.

作者信息

Robinson J P, Schmid M F, Morgan D G, Chiu W

机构信息

Department of Biochemistry, University of Arizona, Tucson 85721.

出版信息

J Mol Biol. 1988 Mar 20;200(2):367-75. doi: 10.1016/0022-2836(88)90247-1.

DOI:10.1016/0022-2836(88)90247-1

PMID:3373534

Abstract

Two-dimensional crystalline arrays of native tetanus toxin have been formed at the interface between a solution of the toxin and a phospholipid monolayer containing a ganglioside. Electron crystallographic analysis has been used to study these periodic arrays. The arrays obey the symmetry of plane group p12(1), with a = 126 A and b = 84 A, and a thickness of 90 A (1 A = 0.1 nm). The three-dimensional structure of tetanus toxin in negative stain is reconstructed to a nominal resolution of 14 A from multiple tilt images. The molecule presents an asymmetric three-lobed structure and could interact with the monolayer in two possible orientations.

摘要

在破伤风毒素溶液与含有神经节苷脂的磷脂单层之间的界面处，已形成天然破伤风毒素的二维晶体阵列。电子晶体学分析已用于研究这些周期性阵列。这些阵列符合平面群p12(1)的对称性，a = 126 Å，b = 84 Å，厚度为90 Å（1 Å = 0.1 nm）。通过多个倾斜图像将负染中破伤风毒素的三维结构重建至标称分辨率14 Å。该分子呈现出不对称的三叶结构，并可能以两种可能的取向与单层相互作用。