从奶牛废水中分离、筛选和综合鉴定生物燃料原料生产候选微藻：一种可持续的方法。
Isolation, screening and comprehensive characterization of candidate microalgae for biofuel feedstock production and dairy effluent treatment: A sustainable approach.
机构信息
Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Prayagraj, U.P., India.
School of Biochemical Engineering, Indian Institute of Technology (BHU) Varanasi, Varanasi, U.P., India.
出版信息
Bioresour Technol. 2019 Dec;293:121998. doi: 10.1016/j.biortech.2019.121998. Epub 2019 Aug 13.
In this study, the indigenous native microalgae were isolated from domestic and dairy effluent (DE) and further screened for DE treatment and lipid accumulation. All the isolated microalgae were examined for their growth adaptability in DE. The growth parameters of 15 isolates were determined and the following six isolates further selected for comprehensive analysis and identified as Desmodesmus sp. ASK01, Chlorella sp. ASK14, Scenedesmus sp. ASK16, Scenedesmus sp. ASK22, Chlorella sp. ASK25 and Chlorella sp. ASK27. The nutrient remediation capacity of six isolates as well as its lipid accumulation potential and biomass composition were determined. The Scenedesmus sp. ASK22 showed the best combined results and promising strain for the DE treatment and biofuel production. Biomass composition of Scenedesmus sp. ASK22 showed an oil accumulation of 30.7% (w/w) and biomass yield 1.22 g L. The fatty acid methyl ester (FAME) mainly composed of C15:0, C16:0, C18:0, C18:1 and C18:3.
在这项研究中，从家庭和奶制品废水（DE）中分离出土著微藻，并进一步筛选用于 DE 处理和脂质积累的微藻。所有分离出的微藻都被检查了其在 DE 中的生长适应性。测定了 15 个分离株的生长参数，进一步选择了以下 6 个分离株进行综合分析，并鉴定为 Desmodesmus sp. ASK01、Chlorella sp. ASK14、Scenedesmus sp. ASK16、Scenedesmus sp. ASK22、Chlorella sp. ASK25 和 Chlorella sp. ASK27。测定了六种分离株的营养修复能力及其脂质积累潜力和生物量组成。Scenedesmus sp. ASK22 表现出最佳的综合结果，是 DE 处理和生物燃料生产的有前途的菌株。Scenedesmus sp. ASK22 的生物量组成显示出 30.7%（w/w）的油脂积累和 1.22g/L 的生物质产量。脂肪酸甲酯（FAME）主要由 C15:0、C16:0、C18:0、C18:1 和 C18:3 组成。
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