The Citrus Research Institute of Zhejiang Province, Taizhou City, Zhejiang Province, 318020, China.
Department of Plant Pathology, University of California, Davis, 95616, CA, USA.
Microb Pathog. 2021 May;154:104845. doi: 10.1016/j.micpath.2021.104845. Epub 2021 Mar 15.
Multiple and consecutive application of fungicide might damage the rhizosphere bacterial community of citrus. In order to evaluated effect of mancozeb on the chemical properties of citrus-cultivated soil and the richness and diversity of rhizosphere bacterial community. The abundance response of rhizosphere bacterial groups without application or with application of 1.333 g mg mancozeb for 2, 4, 6 and 8 times were investigated, and further studied the relationship between the rhizosphere bacterial community and chemical properties of citrus-cultivated soil. We found the rhizosphere bacterial composition and diversity were distinct between soil planted with citrus and without citrus, in addition, the abundance of rhizosphere-associated bacterial species in the soil planted with citrus increased significantly. Meanwhile, the chemical properties and the richness and diversity of rhizosphere bacterial community of the soil planted with citrus did not significantly change among different application frequence of mancozeb. Moreover, with the increased applying times of mancozeb, the relative abundance of Candidatus, Saccharibacteria, Parcubacteria, and Proteobacteria increased but the abundance of Nitrospirae decreased. In our one-year trial, there were less adverse effects of mancozeb on the citrus-cultivated rhizosphere by the repeated application of mancozeb. Therefore, mancozeb, as a fungicide, could be used multiple times to control citrus disease.
杀菌剂的多次连续使用可能会破坏柑橘的根际细菌群落。为了评估代森锰锌对柑橘栽培土壤化学性质以及根际细菌群落丰富度和多样性的影响。研究了在不使用和使用 1.333 g mg 代森锰锌 2、4、6 和 8 次的情况下，根际细菌群的丰度响应，并进一步研究了根际细菌群落与柑橘栽培土壤化学性质之间的关系。我们发现，种植柑橘和不种植柑橘的土壤之间的根际细菌组成和多样性存在明显差异，此外，种植柑橘的土壤中根际相关细菌物种的丰度显著增加。同时，代森锰锌不同施用频率下，柑橘种植土壤的化学性质以及根际细菌群落的丰富度和多样性均无明显变化。此外，随着代森锰锌施用次数的增加，Candidatus、Saccharibacteria、Parcubacteria 和 Proteobacteria 的相对丰度增加，而 Nitrospirae 的丰度降低。在我们为期一年的试验中，代森锰锌的重复使用对柑橘根际的负面影响较小。因此，代森锰锌作为一种杀菌剂，可以多次使用来控制柑橘病害。
