Qian Bei, Li Jialun, Guo Ke, Guo Nengqiang, Zhong Aimei, Yang Jie, Wang Jiecong, Xiao Peng, Sun Jiaming, Xiong Lingyun
Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan 430022, China.
Wuhan Clinical Research Center for Superficial Organ Reconstruction, Wuhan 430022, China.
Regen Biomater. 2021 Mar 13;8(2):rbab003. doi: 10.1093/rb/rbab003. eCollection 2021 Mar.
Associated with persistent oxidative stress, altered inflammatory responses, poor angiogenesis and epithelization, wound healing in diabetic patients is impaired. N-acetylcysteine (NAC) is reported to resist excess reactive oxygen species (ROS) production, prompt angiogenesis and maturation of the epidermis. Studies have revealed that graphene oxide (GO) can regulate cellular behavior and form cross-links with naturally biodegradable polymers such as collagen (COL) to construct composite scaffolds. Here, we reported a COL-based implantable scaffold containing a mixture of GO capable of the sustained delivery of NAC to evaluate the wound healing in diabetic rats. The morphological, physical characteristics, biocompatibility and NAC release profile of the GO-COL-NAC (GCN) scaffold were evaluated . Wound healing studies were performed on a 20 mm dorsal full-skin defect of streptozotocin (STZ)-induced diabetic rats. The injured skin tissue was removed at the 18th day post-surgery for histological analysis and determination of glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD) activity. In diabetic rats, we confirmed that the GCN scaffold presented a beneficial effect in enhancing the wound healing process. Additionally, due to the sustained release of NAC, the scaffold may potentially induce the antioxidant defense system, upregulating the expression levels of the antioxidant enzymes in the wound tissue. The findings revealed that the antioxidant biocompatible composite collagen dressing could not only deliver NAC for ROS inhibition but also promote the wound healing process. This scaffold with valuable therapy potential might enrich the approaches for surgeon in diabetic wound treatment in the future.
与持续的氧化应激、炎症反应改变、血管生成不良和上皮形成受损相关，糖尿病患者的伤口愈合受到损害。据报道，N-乙酰半胱氨酸（NAC）可抵抗过量活性氧（ROS）的产生，促进血管生成和表皮成熟。研究表明，氧化石墨烯（GO）可以调节细胞行为，并与天然可生物降解的聚合物如胶原蛋白（COL）形成交联以构建复合支架。在此，我们报道了一种基于COL的可植入支架，其包含能够持续递送NAC的GO混合物，以评估糖尿病大鼠的伤口愈合情况。对GO-COL-NAC（GCN）支架的形态、物理特性、生物相容性和NAC释放曲线进行了评估。对链脲佐菌素（STZ）诱导的糖尿病大鼠20毫米背部全层皮肤缺损进行伤口愈合研究。在手术后第18天切除受伤的皮肤组织，进行组织学分析并测定谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性。在糖尿病大鼠中，我们证实GCN支架在促进伤口愈合过程中具有有益作用。此外，由于NAC的持续释放，该支架可能潜在地诱导抗氧化防御系统，上调伤口组织中抗氧化酶的表达水平。研究结果表明，这种抗氧化生物相容性复合胶原蛋白敷料不仅可以递送NAC以抑制ROS，还可以促进伤口愈合过程。这种具有宝贵治疗潜力的支架可能会丰富未来外科医生治疗糖尿病伤口的方法。
