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具有 N-乙酰半胱氨酸局部持续释放的多层聚酰胺/胶原蛋白支架促进伤口愈合。

Multi-Layered Polyamide/Collagen Scaffolds with Topical Sustained Release of N-Acetylcysteine for Promoting Wound Healing.

机构信息

Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People's Republic of China.

Wuhan Clinical Research Center for Superficial Organ Reconstruction, Wuhan 430022, China.

出版信息

Int J Nanomedicine. 2020 Feb 28;15:1349-1361. doi: 10.2147/IJN.S232190. eCollection 2020.

DOI:10.2147/IJN.S232190
PMID:32184590
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7053812/
Abstract

BACKGROUND

Impaired wound healing might be associated with many issues, especially overactive of reactive oxygen species (ROS), deficiency of blood vessels and immature of epidermis. N-acetylcysteine (NAC), as an antioxidant, could solve these problems by inhibiting overreactive of ROS, promoting revascularization and accelerating re-epithelialization. How to deliver NAC in situ with a controllable releasing speed still remain a challenge.

MATERIALS AND METHODS

In this study, we combined collagen (Col) with N-acetylcysteine to perform the characteristics of sustained release and chemically crosslinked Col/NAC composite with polyamide (PA) nanofibers to enhance the mechanical property of collagen and fabricated this multi-layered scaffold (PA-Col/NAC scaffold). The physical properties of the scaffolds such as surface characteristics, water absorption and tensile modulus were tested. Meanwhile, the ability to promote wound healing in vitro and in vivo were investigated.

RESULTS

These scaffolds were porous and performed great water absorption. The PA-Col/NAC scaffold could sustainably release NAC for at least 14 days. After cell implantation, PA-Col/NAC scaffold showed better cell proliferation and cell migration than the other groups. In vivo, PA-Col/NAC scaffolds could promote wound healing best among all the groups.

CONCLUSION

The multi-layered scaffolds could obviously accelerate the process of wound healing and exert better and prolonged effects.

摘要

背景

伤口愈合受损可能与许多问题有关，特别是活性氧（ROS）过度活跃、血管不足和表皮不成熟。N-乙酰半胱氨酸（NAC）作为一种抗氧化剂，可以通过抑制 ROS 的过度反应、促进再血管化和加速再上皮化来解决这些问题。如何以可控的释放速度原位输送 NAC 仍然是一个挑战。

材料和方法

在这项研究中，我们将胶原（Col）与 N-乙酰半胱氨酸结合，以实现持续释放的特性，并使用聚酰胺（PA）纳米纤维对 Col/NAC 复合材料进行化学交联，以增强胶原的机械性能，并制造出这种多层支架（PA-Col/NAC 支架）。测试了支架的物理性质，如表面特性、吸水性和拉伸模量。同时，研究了其在体外和体内促进伤口愈合的能力。

结果

这些支架是多孔的，具有很好的吸水性。PA-Col/NAC 支架至少可以持续释放 NAC 14 天。细胞植入后，PA-Col/NAC 支架的细胞增殖和细胞迁移能力均优于其他组。在体内，PA-Col/NAC 支架在所有组中能最好地促进伤口愈合。

结论

多层支架可以明显加速伤口愈合过程，并发挥更好和更持久的效果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/75a24f02fb3b/IJN-15-1349-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/d5bfec23b629/IJN-15-1349-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/ef1e5605980c/IJN-15-1349-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/ddaf6350b855/IJN-15-1349-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/218ac51b0f91/IJN-15-1349-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/d36c31d626ce/IJN-15-1349-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/629ed131424b/IJN-15-1349-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/f19256dfb7e2/IJN-15-1349-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/75a24f02fb3b/IJN-15-1349-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/d5bfec23b629/IJN-15-1349-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/ef1e5605980c/IJN-15-1349-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/ddaf6350b855/IJN-15-1349-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/218ac51b0f91/IJN-15-1349-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/d36c31d626ce/IJN-15-1349-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/629ed131424b/IJN-15-1349-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/f19256dfb7e2/IJN-15-1349-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0ba/7053812/75a24f02fb3b/IJN-15-1349-g0008.jpg

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