Tanhaei Mohammad, Mohebbi Seyed Reza, Hosseini Seyed Masoud, Rafieepoor Maedeh, Kazemian Shabnam, Ghaemi Amir, Shamloei Sharareh, Mirjalali Hamed, Asadzadeh Aghdaei Hamid, Zali Mohammad Reza
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Arabi str., Yaman Ave., Velenjak, Tehran, Iran.
Environ Sci Pollut Res Int. 2021 Aug;28(29):38629-38636. doi: 10.1007/s11356-021-13393-9. Epub 2021 Mar 18.
Following the official announcement of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) worldwide pandemic spread by WHO on March 11, 2020, more than 300,000 COVID-19 cases reported in Iran resulting in approximately 17,000 deaths as of August 2, 2020. In the present survey, we investigated the presence of SARS-CoV-2 RNA in raw and treated wastewater samples in Tehran, Iran. Untreated and treated wastewater samples were gathered from four wastewater treatment plants over a month period from June to July 2020. Firstly, an adsorption-elution concentration method was tested using an avian coronavirus (infectious bronchitis virus, IBV). Then, the method was effectively employed to survey the presence of SARS-CoV-2 genome in influent and effluent wastewater samples. SARS-CoV-2 RNA was found in 8 out of 10 treated wastewater samples utilizing a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) test to detect ORF1ab and N genes. Moreover, the rate of positivity in wastewater samples increased in last sample collection that shows circulation of SARS-CoV-2 was increased among the population. In addition, the high values detected in effluent wastewater from local wastewater treatment plants have several implications in health and ecology that should be further assessed.
2020年3月11日世界卫生组织正式宣布严重急性呼吸综合征冠状病毒2（SARS-CoV-2）在全球大流行后，截至2020年8月2日，伊朗报告了超过30万例新冠肺炎病例，导致约1.7万人死亡。在本次调查中，我们研究了伊朗德黑兰未经处理和经处理的废水样本中SARS-CoV-2 RNA的存在情况。2020年6月至7月期间，在一个月的时间里，从四个污水处理厂采集了未经处理和经处理的废水样本。首先，使用禽冠状病毒（传染性支气管炎病毒，IBV）测试了一种吸附-洗脱浓缩方法。然后，该方法被有效地用于调查进水和出水废水样本中SARS-CoV-2基因组的存在情况。利用逆转录定量聚合酶链反应（RT-qPCR）检测ORF1ab和N基因，在10个经处理的废水样本中有8个检测到SARS-CoV-2 RNA。此外，在最后一次样本采集时，废水样本中的阳性率有所上升，这表明SARS-CoV-2在人群中的传播有所增加。此外，当地污水处理厂出水检测到的高值对健康和生态有若干影响，应进一步评估。
