Key Laboratory of Functional Polymer Materials (Ministry of Education), Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing 100190, China.
Nano Lett. 2021 Apr 14;21(7):2793-2799. doi: 10.1021/acs.nanolett.0c04825. Epub 2021 Mar 19.
Despite progress on DNA-assembled nanoparticle (NP) superstructures, their complicated synthesis procedures hamper their potential biomedical applications. Here, we present an exceptionally simple strategy for the synthesis of single-stranded DNA (ssDNA) assembled FeO supraparticles (DFe-SPs) as magnetic resonance contrast agents. Unlike traditional approaches that assemble DNA-conjugated NPs via Watson-Crick hybridization, our DFe-SPs are formed with a high yield through one-step synthesis and assembly of ultrasmall FeO NPs via ssDNA-metal coordination bridges. We demonstrate that the DFe-SPs can efficiently accumulate into tumors for sensitive MR imaging. By virtue of reversible DNA-metal coordination bridges, the DFe-SPs could be disassembled into isolated small NPs in vivo, facilitating their elimination from the body. This work opens a new avenue for the ssDNA-mediated synthesis of superstructures, which expands the repertoire of DNA-directed NP assembly for biomedical applications.
尽管在 DNA 组装纳米颗粒 (NP) 超结构方面取得了进展，但它们复杂的合成程序阻碍了它们在生物医学中的潜在应用。在这里，我们提出了一种非常简单的策略，用于合成单链 DNA (ssDNA) 组装的 FeO 超粒子 (DFe-SP) 作为磁共振对比剂。与传统的通过 Watson-Crick 杂交将 DNA 偶联的 NPs 组装的方法不同，我们的 DFe-SP 通过一步合成和 ssDNA-金属配位桥组装超小的 FeO NPs 以高产率形成。我们证明 DFe-SP 可以有效地聚集到肿瘤中进行敏感的磁共振成像。由于可逆的 DNA-金属配位桥，DFe-SP 可以在体内解组装成孤立的小 NPs，有利于它们从体内排出。这项工作为 ssDNA 介导的超结构合成开辟了新途径，扩展了 DNA 指导的 NP 组装在生物医学中的应用范围。
