Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, Republic of Korea.
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, Republic of Korea.
Biochem Biophys Res Commun. 2021 Apr 30;551:140-147. doi: 10.1016/j.bbrc.2021.03.019. Epub 2021 Mar 16.
Cell migration is a complex and important process in cancer progression. Vimentin has pivotal roles in cancer cell migration, and various signaling pathways including the AKT pathway are involved in cancer cell migration via vimentin regulation. Recent studies have revealed that voltage-gated potassium (Kv) channels have important functions in cancer cell migration; however, the exact mechanism is still unclear. In the present study, we focused on Kv3 channels with vimentin in cancer migration using human cervical cancer cells (HeLa) and canine mammary tumor cells (CHMp). Cancer cell migration was significantly inhibited, and vimentin expression was significantly decreased by Kv3 blocker, BDS-II. The Kv3 blocker also inactivated the AKT pathway in HeLa cells. In addition, reduced expressions of vimentin and Kv3.4 were observed in HeLa cells when treated with AKT blocker, MK2206. These results suggest that Kv3 channels play important roles in cancer cell migration by regulating vimentin and having closely related with the AKT pathway in human cervical cancer cells.
细胞迁移是癌症进展中一个复杂而重要的过程。波形蛋白在癌细胞迁移中起着关键作用，各种信号通路，包括 AKT 通路，通过波形蛋白的调节参与癌细胞迁移。最近的研究表明，电压门控钾（Kv）通道在癌细胞迁移中具有重要功能；然而，确切的机制尚不清楚。在本研究中，我们使用人宫颈癌（HeLa）细胞和犬乳腺肿瘤（CHMp）细胞专注于与波形蛋白相关的 Kv3 通道在癌症迁移中的作用。Kv3 阻断剂 BDS-II 显著抑制了癌细胞的迁移，并显著降低了波形蛋白的表达。Kv3 阻断剂还使 HeLa 细胞中的 AKT 通路失活。此外，当用 AKT 阻断剂 MK2206 处理 HeLa 细胞时，观察到波形蛋白和 Kv3.4 的表达减少。这些结果表明，Kv3 通道通过调节波形蛋白在癌细胞迁移中发挥重要作用，并且与人类宫颈癌细胞中的 AKT 通路密切相关。
