Division of Infection and Immunity, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
Commun Biol. 2021 Mar 19;4(1):377. doi: 10.1038/s42003-021-01901-z.
Mammalian three-dimensional (3D) enteroids mirror in vivo intestinal organisation and are powerful tools to investigate intestinal cell biology and host-pathogen interactions. We have developed complex multilobulated 3D chicken enteroids from intestinal embryonic villi and adult crypts. These avian enteroids develop optimally in suspension without the structural support required to produce mammalian enteroids, resulting in an inside-out enteroid conformation with media-facing apical brush borders. Histological and transcriptional analyses show these enteroids comprise of differentiated intestinal epithelial cells bound by cell-cell junctions, and notably, include intraepithelial leukocytes and an inner core of lamina propria leukocytes. The advantageous polarisation of these enteroids has enabled infection of the epithelial apical surface with Salmonella Typhimurium, influenza A virus and Eimeria tenella without the need for micro-injection. We have created a comprehensive model of the chicken intestine which has the potential to explore epithelial and leukocyte interactions and responses in host-pathogen, food science and pharmaceutical research.
哺乳动物三维 (3D) 类器官模拟了体内肠道组织，是研究肠道细胞生物学和宿主-病原体相互作用的强大工具。我们已经从肠胚绒毛和成年隐窝中开发出复杂的多叶状鸡 3D 类肠器官。这些禽类类肠器官在悬浮状态下最佳发育，不需要产生哺乳动物类肠器官所需的结构支撑，从而形成具有面向培养基的顶端刷状边界的内外翻类肠器官构象。组织学和转录分析表明，这些类肠器官由细胞-细胞连接结合的分化肠上皮细胞组成，值得注意的是，还包括上皮内白细胞和固有层白细胞的核心。这些类肠器官的有利极化使得能够对上皮细胞的顶端表面进行感染，例如用沙门氏菌 Typhimurium、甲型流感病毒和艾美球虫进行感染，而无需微注射。我们已经创建了一个鸡肠的综合模型，该模型具有探索宿主-病原体、食品科学和药物研究中上皮细胞和白细胞相互作用和反应的潜力。
