Flavisolibacter longurius sp. nov., isolated from soil.

A novel Gram-negative bacterial strain BT320 was isolated from soil collected in Uijeongbu city, Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BT320 belong to a distinct lineage within the genus Flavisolibacter (family Chitinophagaceae, order Chitinophagales, class Chitinophagia). The strain BT320 was closely related to Flavisolibacter galbus 17J28-26 (97.6% 16S rRNA gene similarity), Flavisolibacter nicotianae X7X (96.7%), Flavisolibacter ginsengiterrae Gsoil 492 (96.2%), and Flavisolibacter ginsengisoli Gsoil 643 (96.1%). The genome size of strain BT320 was 5,664,094 bp. Bacterial growth was observed at 10-37 °C (optimum 25 °C) and pH 6.0-8.0 (optimum pH 7.0) on R2A agar. The major cellular fatty acids of strain BT320 were iso-C, summed feature 3 (C ω6c/C ω7c), and summed feature 1 (iso-C H/C 3OH). Its predominant respiratory quinone was MK-7. The major polar lipid of strain BT320 was identified to be phosphatidylethanolamine (PE). Based on the biochemical, chemotaxonomic, and phylogenetic analysis, strain BT320 can be suggested as a novel bacterial species within the genus Flavisolibacter and the proposed name is Flavisolibacter longurius. The type strain of Flavisolibacter longurius is BT320 (= KCTC 72422 = NBRC 114375).