Kramer J K, Farnworth E R, Thompson B K, Corner A H
Animal Research Centre, Agriculture Canada, Ottawa.
Lipids. 1988 Mar;23(3):199-206. doi: 10.1007/BF02535458.
Male, female and castrated rats, three wk of age, were fed a low-fat diet for 14 wk followed by high-fat diets (20% by weight) for one wk containing graded levels of erucic acid from 1 to 50%, to evaluate the effect of short-term feeding and interaction of male sex hormones on formation of heart lesions. Some rats within each group were returned to the low-fat diet for one wk after the test period. For comparison, one group of three-wk-old male rats was fed the high fat 50% erucic acid diet for 15 wk. Erucic acid depressed growth rate and food consumption and increased cardiac lipidosis and triglycerides proportional to the erucic acid content of the diet. There were no sex differences, and the effects disappeared once rats were returned to the low-fat diet for one week. There was a significance (P less than 0.05) in the incidence of myocardial necrosis among male rats fed increased levels of erucic acid for one week, but the response was not linear to the increase in dietary erucic acid. Furthermore, the response was much less than in males fed the 50% erucic acid diet continually for 15 weeks. These results suggest that the short-term model is not a suitable substitute for the long-term feeding trial to test the cardiopathogenicity of a vegetable oil. The significantly lower incidence in myocardial lesions in female and castrated male rats compared with male rats suggests involvement of sex hormones.(ABSTRACT TRUNCATED AT 250 WORDS)
对3周龄的雄性、雌性和去势大鼠喂食低脂饮食14周，随后喂食含1%至50%不同芥酸水平的高脂饮食（按重量计20%）1周，以评估短期喂食和雄性性激素相互作用对心脏病变形成的影响。每组中的一些大鼠在试验期后恢复低脂饮食1周。作为对照，一组3周龄雄性大鼠喂食含50%芥酸菜酸的高脂饮食15周。芥酸降低生长速率和食物消耗量，并增加心脏脂肪变性和甘油三酯，且与饮食中的芥酸含量成正比。不存在性别差异，且大鼠恢复低脂饮食1周后这些影响就会消失。喂食增加水平芥酸1周的雄性大鼠中心肌坏死发生率存在显著差异（P小于0.05），但该反应与饮食中芥酸增加不成线性关系。此外，该反应远小于持续喂食含50%芥酸菜酸饮食15周的雄性大鼠。这些结果表明，短期模型并非测试植物油心脏致病性的长期喂食试验的合适替代方法。与雄性大鼠相比，雌性和去势雄性大鼠中心肌病变发生率显著较低，提示性激素参与其中。（摘要截选于250字）
