Ashy A A, Salleh M, Ardawi M
Department of Surgery, College of Medicine, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
Metabolism. 1988 Jun;37(6):602-9. doi: 10.1016/0026-0495(88)90179-5.
Suspensions of metabolically viable human small-intestinal epithelial cells (enterocytes) were used to assess the metabolism of substrate(s) normally derived from the blood circulation to the intestinal mucosa (glucose, glutamine, and ketone bodies). Glutamine, glutamate, and glucose were the only substrates that caused major increases in oxygen consumption by isolated human enterocytes. In human enterocytes 72% of glucose could be accounted for as lactate. Human enterocytes utilized glutamine at about 14.90 mumol/min/g dry wt, with glutamate, alanine, aspartate, and ammonia as the major end-products. Human enterocytes utilized 3-hydroxybutyrate and acetoacetate at similar rates. Under the experimental condition employed, the respiratory fuels of human enterocytes include glutamine, glucose, and to a lesser extent, ketone bodies as indicated in the proportion of oxygen consumption attributed to these fuels and to the extent of utilization.
利用代谢活性人类小肠上皮细胞（肠细胞）悬浮液来评估通常从血液循环进入肠黏膜的底物（葡萄糖、谷氨酰胺和酮体）的代谢情况。谷氨酰胺、谷氨酸和葡萄糖是仅有的能使分离出的人类肠细胞耗氧量大幅增加的底物。在人类肠细胞中，72%的葡萄糖可转化为乳酸。人类肠细胞以约14.90微摩尔/分钟/克干重的速率利用谷氨酰胺，主要终产物为谷氨酸、丙氨酸、天冬氨酸和氨。人类肠细胞以相似的速率利用3-羟基丁酸和乙酰乙酸。在所采用的实验条件下，人类肠细胞的呼吸燃料包括谷氨酰胺、葡萄糖，以及在较小程度上的酮体，这可从归因于这些燃料的耗氧量比例和利用程度看出。
