Moradi Niknaz, Bidgoli Sepideh Arbabi, Chaichian Shahla
Faculty of Pharmacy and Pharmaceutical Sciences, Islamic Azad University, Tehran Medical Sciences University, Tehran, Iran.
Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Islamic Azad University, Tehran Medical Sciences University, Tehran, Iran.
Obstet Gynecol Sci. 2021 May;64(3):274-283. doi: 10.5468/ogs.20094. Epub 2021 Mar 19.
Hormonal and inflammatory mechanisms are involved in the pathogenesis of polycystic ovarian syndrome (PCOS), which is a prevalent metabolic disorder among women of reproductive age. We aimed to evaluate the comparative efficiency of short-term oral administration of Korean red ginseng extract (KRGE) and the standard treatment on PCOS by focusing on the histopathological parameters and serum levels of luteinizing hormone (LH), folliclestimulating hormone (FSH), testosterone, and nuclear factor kappa B (NF-κB).
A PCOS rat model was established by oral gavage of letrozole (1 mg/kg) for 21 days. The serum levels of LH, FSH, testosterone, and NF-κB were measured, and the morphological features and differences of the ovaries were examined in each group using a light microscope before and after 14 days of treatment with oral regimens-KRGE, oral contraceptives (OCPs), KRGE+OCPs, and carboxymethyl cellulose (CMC).
OCPs alone could not normalize the mean ovarian weights of PCOS rats despite the 14-day oral regimen, but they were more effective in reducing the number and size of cysts compared to others. KRGE alone and in combination with OCPs was effective in normalizing abnormal ovarian weights, decreasing LH serum levels, and dissipating the ovarian cysts in PCOS rats. However, when combined with the standard regimen, KRGE showed additional therapeutic effects by efficiently reducing serum testosterone and NF-κB levels.
Our necropsy and histopathological evidence suggests the efficacy of KRGE as a novel integrative medicine against abnormal multiple follicular cysts. However, antiandrogenic and anti-inflammatory effects were only seen in animals that were administered a combination of KRGE and the standard regimen.
激素和炎症机制参与多囊卵巢综合征（PCOS）的发病过程，PCOS是育龄女性中一种常见的代谢紊乱疾病。我们旨在通过关注组织病理学参数以及黄体生成素（LH）、促卵泡生成素（FSH）、睾酮和核因子κB（NF-κB）的血清水平，评估短期口服韩国红参提取物（KRGE）与标准治疗对PCOS的相对疗效。
通过口服来曲唑（1毫克/千克）21天建立PCOS大鼠模型。测量LH、FSH、睾酮和NF-κB的血清水平，并在口服KRGE、口服避孕药（OCPs）、KRGE + OCPs和羧甲基纤维素（CMC）治疗14天前后，使用光学显微镜检查每组卵巢的形态特征和差异。
尽管进行了14天的口服治疗，但单独使用OCPs并不能使PCOS大鼠的平均卵巢重量恢复正常，但与其他药物相比，它们在减少囊肿数量和大小方面更有效。单独使用KRGE以及与OCPs联合使用，均可有效使PCOS大鼠异常的卵巢重量恢复正常，降低LH血清水平，并消散卵巢囊肿。然而，当与标准治疗方案联合使用时，KRGE通过有效降低血清睾酮和NF-κB水平显示出额外的治疗效果。
我们的尸检和组织病理学证据表明，KRGE作为一种新型综合药物对异常多发性滤泡囊肿具有疗效。然而，抗雄激素和抗炎作用仅在同时给予KRGE和标准治疗方案的动物中观察到。
