National Engineering Research Center for Environment-Friendly Metallurgy in Producing Premium Non-Ferrous Metals, GRINM Group Co., Ltd, Beijing, 101407, China.
School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Bioprocess Biosyst Eng. 2023 Jun;46(6):851-865. doi: 10.1007/s00449-023-02869-2. Epub 2023 Apr 10.
Aromatic volatile organic compounds (VOCs) are a type of common pollution form in chemical contaminated sites. In this study, seven aromatic VOCs such as benzene, toluene, ethylbenzene, chlorobenzene, m-xylene, p-chlorotoluene and p-chlorotrifluorotoluene were used as the only carbon source, and four strains of highly efficient degrading bacteria were screened from the soil of chemical contaminated sites, then the synthetic bacterial consortium was constructed after mixing with an existing functional strain (Bacillus benzoevorans) preserved in the laboratory. After that, the synthetic bacterial consortium was used to explore the degradation effect of simulated aromatic VOCs polluted wastewater. The results showed that the functional bacterium could metabolize with aromatic VOCs as the only carbon source and energy. Meanwhile, the growth of the synthetic bacterial consortium increased with the additional carbon resources and the alternative of organic nitrogen source. Ultimately, the applicability of the synthetic bacterial consortium in organic contaminated sites was explored through the study of broad-spectrum activity.
芳香族挥发性有机化合物（VOCs）是化学污染场地中常见的污染形式之一。本研究以苯、甲苯、乙苯、氯苯、间二甲苯、对氯甲苯和对氯三氟甲苯等 7 种芳香族 VOCs 作为唯一碳源，从化学污染场地土壤中筛选出 4 株高效降解菌，然后与实验室保藏的现有功能菌（苯并恶唑芽孢杆菌）混合构建合成细菌共生体。之后，采用该合成细菌共生体来探索模拟芳香族 VOCs 污染废水中的降解效果。结果表明，功能菌可以利用芳香族 VOCs 作为唯一的碳源和能源进行代谢。同时，随着外加碳源和有机氮源的替代，合成细菌共生体的生长也随之增加。最终，通过广谱活性研究探索了合成细菌共生体在有机污染场地中的适用性。
