Endo Yushiro, Fukui Shoichi, Koga Tomohiro, Sasaki Daisuke, Hasegawa Hiroo, Yanagihara Katsunori, Okayama Akihiko, Nakamura Tatsufumi, Kawakami Atsushi, Nakamura Hideki
Department of Immunology and Rheumatology, Division of Advanced Preventive Medical Sciences, Nagasaki University Graduate School of Medical Sciences, Nagasaki, Japan.
Department of Laboratory Medicine, Nagasaki University Hospital, Nagasaki, Japan.
J Int Med Res. 2021 Mar;49(3):3000605211002083. doi: 10.1177/03000605211002083.
It remains unclear whether human T-cell leukemia virus type 1 (HTLV-1) infection influences therapeutic responses in patients with rheumatic diseases and whether immunosuppressive treatments increase the risk of HTLV-1-related complications in HTLV-1 carriers with rheumatic diseases. We examined the effects of tocilizumab (TCZ), an interleukin (IL)-6 receptor antagonist, on two HTLV-1-infected T-cell lines (HCT-5 and MT-2) .
We evaluated production of cytokines and chemokines, expression of HTLV-I associated genes, HTLV-1 proviral load (PVL), expression of HTLV-1 structural proteins, and apoptosis.
There were no significant differences in cytokine and chemokine levels in the culture supernatants of HCT-5 and MT-2 cells treated with phosphate-buffered saline (PBS) or TCZ. No significant differences were detected in mRNA abundance of Tax or HBZ, PVL, expression of the HTLV-1 structural protein GAG, or apoptosis among HCT-5 and MT-2 cells treated with PBS or TCZ.
TCZ had no effect the cytokine profiles, HTLV-1 gene and protein expression, PVL, or apoptosis in HTLV-1-infected T-cell lines. Thus, TCZ treatment has no effect on HTLV-1 infection .
目前尚不清楚人类1型T细胞白血病病毒（HTLV-1）感染是否会影响风湿性疾病患者的治疗反应，以及免疫抑制治疗是否会增加患有风湿性疾病的HTLV-1携带者发生HTLV-1相关并发症的风险。我们研究了白细胞介素（IL）-6受体拮抗剂托珠单抗（TCZ）对两种HTLV-1感染的T细胞系（HCT-5和MT-2）的影响。
我们评估了细胞因子和趋化因子的产生、HTLV-I相关基因的表达、HTLV-1前病毒载量（PVL）、HTLV-1结构蛋白的表达以及细胞凋亡情况。
用磷酸盐缓冲盐水（PBS）或TCZ处理的HCT-5和MT-2细胞培养上清液中的细胞因子和趋化因子水平没有显著差异。在用PBS或TCZ处理的HCT-5和MT-2细胞中，Tax或HBZ的mRNA丰度、PVL、HTLV-1结构蛋白GAG的表达或细胞凋亡均未检测到显著差异。
TCZ对HTLV-1感染的T细胞系中的细胞因子谱、HTLV-1基因和蛋白表达、PVL或细胞凋亡没有影响。因此，TCZ治疗对HTLV-1感染没有作用。
