TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin, China.
PLoS One. 2013 Jul 29;8(7):e70329. doi: 10.1371/journal.pone.0070329. Print 2013.
We have sequenced the gene clusters for type strains of the Acinetobacter baumannii serotyping scheme developed in the 1990s, and used the sequences to better understand diversity in surface polysaccharides of the genus. We obtained genome sequences for 27 available serovar type strains, and identified 25 polysaccharide gene cluster sequences. There are structures for 12 of these polysaccharides, and in general the genes present are appropriate to the structure where known. This greatly facilitates interpretation. We also find 53 different glycosyltransferase genes, and for 7 strains can provisionally allocate specific genes to all linkages. We identified primers that will distinguish the 25 sequence forms by PCR or microarray, or alternatively the genes can be used to determine serotype by "molecular serology". We applied the latter to 190 Acinetobacter genome-derived gene-clusters, and found 76 that have one of the 25 gene-cluster forms. We also found novel gene clusters and added 52 new gene-cluster sequence forms with different wzy genes and different gene contents. Altogether, the strains that have one of the original 25 sequence forms include 98 A. baumannii (24 from our strains) and 5 A. nosocomialis (3 from our strains), whereas 32 genomes from 12 species other than A. baumannii or A. nosocomialis, all have new sequence forms. One of the 25 serovar type sequences is found to be in European clone I (EC I), 2 are in EC II but none in EC III. The public genome strains add an additional 52 new sequence forms, and also bring the number found in EC I to 5, in EC II to 9 and in EC III to 2.
我们已经对 20 世纪 90 年代制定的鲍曼不动杆菌血清分型方案的典型菌株的基因簇进行了测序，并利用这些序列更好地了解了该属表面多糖的多样性。我们获得了 27 个现有血清型菌株的基因组序列，并鉴定了 25 个多糖基因簇序列。其中有 12 种多糖的结构，并且通常已知的结构都有相应的基因存在。这极大地促进了对多糖结构的解读。我们还发现了 53 个不同的糖基转移酶基因，对于 7 株菌，可以暂时将特定基因分配到所有键合点上。我们鉴定了一些引物，可以通过 PCR 或微阵列将这 25 种序列形式区分开来，或者可以使用这些基因通过“分子血清学”来确定血清型。我们将后一种方法应用于 190 个源自不动杆菌基因组的基因簇，发现其中 76 个具有 25 种基因簇形式之一。我们还发现了新的基因簇，并添加了 52 种具有不同 wzy 基因和不同基因含量的新基因簇序列形式。总的来说，具有原始 25 种序列形式之一的菌株包括 98 株鲍曼不动杆菌（其中 24 株来自我们的菌株）和 5 株鲍曼不动杆菌 nosocomialis（其中 3 株来自我们的菌株），而 12 种除鲍曼不动杆菌或鲍曼不动杆菌 nosocomialis 以外的其他种的 32 个基因组都具有新的序列形式。其中一个血清型序列被发现存在于欧洲克隆 I（EC I）中，2 个存在于 EC II 中，但在 EC III 中不存在。公共基因组菌株增加了另外 52 种新的序列形式，并且还将在 EC I 中发现的数量增加到 5，在 EC II 中增加到 9，在 EC III 中增加到 2。
