文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

基于甘露糖偶联壳聚糖的流感纳米疫苗在母体抗体阳性猪中的免疫原性和保护效力。

Immunity and Protective Efficacy of Mannose Conjugated Chitosan-Based Influenza Nanovaccine in Maternal Antibody Positive Pigs.

机构信息

Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Wooster, OH, United States.

Department of Veterinary Preventive Medicine, Wooster, OH, United States.

出版信息

Front Immunol. 2021 Mar 4;12:584299. doi: 10.3389/fimmu.2021.584299. eCollection 2021.

DOI:10.3389/fimmu.2021.584299
PMID:33746943
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7969509/
Abstract

Parenteral administration of killed/inactivated swine influenza A virus (SwIAV) vaccine in weaned piglets provides variable levels of immunity due to the presence of preexisting virus specific maternal derived antibodies (MDA). To overcome the effect of MDA on SwIAV vaccine in piglets, we developed an intranasal deliverable killed SwIAV antigen (KAg) encapsulated chitosan nanoparticles called chitosan-based NPs encapsulating KAg (CS NPs-KAg) vaccine. Further, to target the candidate vaccine to dendritic cells and macrophages which express mannose receptor, we conjugated mannose to chitosan (mCS) and formulated KAg encapsulated mCS nanoparticles called mannosylated chitosan-based NPs encapsulating KAg (mCS NPs-KAg) vaccine. In MDA-positive piglets, prime-boost intranasal inoculation of mCS NPs-KAg vaccine elicited enhanced homologous (H1N2-OH10), heterologous (H1N1-OH7), and heterosubtypic (H3N2-OH4) influenza virus-specific secretory IgA (sIgA) antibody response in nasal passage compared to CS NPs-KAg vaccinates. In vaccinated upon challenged with a heterologous SwIAV H1N1, both mCS NPs-KAg and CS NPs-KAg vaccinates augmented H1N2-OH10, H1N1-OH7, and H3N2-OH4 virus-specific sIgA antibody responses in nasal swab, lung lysate, and bronchoalveolar lavage (BAL) fluid; and IgG antibody levels in lung lysate and BAL fluid samples. Whereas, the multivalent commercial inactivated SwIAV vaccine delivered intramuscularly increased serum IgG antibody response. In mCS NPs-KAg and CS NPs-KAg vaccinates increased H1N2-OH10 but not H1N1-OH7 and H3N2-OH4-specific serum hemagglutination inhibition titers were observed. Additionally, mCS NPs-KAg vaccine increased specific recall lymphocyte proliferation and cytokines IL-4, IL-10, and IFNγ gene expression compared to CS NPs-KAg and commercial SwIAV vaccinates in tracheobronchial lymph nodes. Consistent with the immune response both mCS NPs-KAg and CS NPs-KAg vaccinates cleared the challenge H1N1-OH7 virus load in upper and lower respiratory tract more efficiently when compared to commercial vaccine. The virus clearance was associated with reduced gross lung lesions. Overall, mCS NP-KAg vaccine intranasal immunization in MDA-positive pigs induced a robust cross-reactive immunity and offered protection against influenza virus.

摘要

经口给予已杀死/灭活的猪流感 A 病毒（SwIAV）疫苗会在断奶仔猪中产生可变水平的免疫，这是由于存在预先存在的病毒特异性母体衍生抗体（MDA）。为了克服 MDA 对仔猪中 SwIAV 疫苗的影响，我们开发了一种可经鼻给予的壳聚糖纳米颗粒包裹的已杀死 SwIAV 抗原（KAg），称为壳聚糖基纳米颗粒包裹 KAg（CS NPs-KAg）疫苗。此外，为了将候选疫苗靶向表达甘露糖受体的树突状细胞和巨噬细胞，我们将甘露糖与壳聚糖（mCS）缀合，并制定了壳聚糖基纳米颗粒包裹 KAg（mCS NPs-KAg）疫苗。在 MDA 阳性的仔猪中，经鼻接种 mCS NPs-KAg 疫苗进行初免-加强免疫，可引起鼻道中增强的同源（H1N2-OH10）、异源（H1N1-OH7）和异亚种（H3N2-OH4）流感病毒特异性分泌型 IgA（sIgA）抗体反应，而 CS NPs-KAg 疫苗则不能。在接种疫苗后用异源 SwIAV H1N1 进行攻毒，mCS NPs-KAg 和 CS NPs-KAg 疫苗均增强了 H1N2-OH10、H1N1-OH7 和 H3N2-OH4 病毒特异性 sIgA 抗体反应，在鼻拭子、肺裂解物和支气管肺泡灌洗液（BAL）中；并且在肺裂解物和 BAL 样本中增加了 IgG 抗体水平。而肌肉内接种多价市售 SwIAV 灭活疫苗可增加血清 IgG 抗体反应。在 mCS NPs-KAg 和 CS NPs-KAg 疫苗接种中，观察到增强的 H1N2-OH10 但没有 H1N1-OH7 和 H3N2-OH4 特异性血清血凝抑制滴度。此外，与 CS NPs-KAg 和市售 SwIAV 疫苗相比，mCS NPs-KAg 疫苗增加了气管支气管淋巴结中特定的回忆性淋巴细胞增殖和细胞因子 IL-4、IL-10 和 IFNγ 基因表达。与免疫反应一致，与市售疫苗相比，mCS NPs-KAg 和 CS NPs-KAg 疫苗接种更有效地清除了上呼吸道和下呼吸道中的挑战 H1N1-OH7 病毒载量。病毒清除与肺部大体病变减少有关。总的来说，mCS NP-KAg 疫苗经鼻免疫接种 MDA 阳性猪可诱导出强大的交叉反应性免疫，并提供对流感病毒的保护。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8433/7969509/b02f0d773c75/fimmu-12-584299-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8433/7969509/fd4ae97db9e8/fimmu-12-584299-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8433/7969509/82fb219a2685/fimmu-12-584299-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8433/7969509/07bab4796921/fimmu-12-584299-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8433/7969509/b02f0d773c75/fimmu-12-584299-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8433/7969509/fd4ae97db9e8/fimmu-12-584299-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8433/7969509/82fb219a2685/fimmu-12-584299-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8433/7969509/07bab4796921/fimmu-12-584299-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8433/7969509/b02f0d773c75/fimmu-12-584299-g0004.jpg

相似文献

[1]
Immunity and Protective Efficacy of Mannose Conjugated Chitosan-Based Influenza Nanovaccine in Maternal Antibody Positive Pigs.

Front Immunol. 2021

[2]
Mucosal Immunity and Protective Efficacy of Intranasal Inactivated Influenza Vaccine Is Improved by Chitosan Nanoparticle Delivery in Pigs.

Front Immunol. 2018-5-2

[3]
Intranasal Delivery of Inactivated Influenza Virus and Poly(I:C) Adsorbed Corn-Based Nanoparticle Vaccine Elicited Robust Antigen-Specific Cell-Mediated Immune Responses in Maternal Antibody Positive Nursery Pigs.

Front Immunol. 2020

[4]
Polyanhydride nanovaccine against swine influenza virus in pigs.

Vaccine. 2017-2-22

[5]
Poly(I:C) augments inactivated influenza virus-chitosan nanovaccine induced cell mediated immune response in pigs vaccinated intranasally.

Vet Microbiol. 2020-2-13

[6]
Biodegradable nanoparticle delivery of inactivated swine influenza virus vaccine provides heterologous cell-mediated immune response in pigs.

J Control Release. 2017-1-2

[7]
A split influenza vaccine formulated with a combination adjuvant composed of alpha-D-glucan nanoparticles and a STING agonist elicits cross-protective immunity in pigs.

J Nanobiotechnology. 2022-11-11

[8]
Evaluation of CpG-ODN-adjuvanted polyanhydride-based intranasal influenza nanovaccine in pigs.

Vet Microbiol. 2019-8-28

[9]
Poly I:C adjuvanted inactivated swine influenza vaccine induces heterologous protective immunity in pigs.

Vaccine. 2015-1-15

[10]
Divergent immune responses and disease outcomes in piglets immunized with inactivated and attenuated H3N2 swine influenza vaccines in the presence of maternally-derived antibodies.

Virology. 2014-7-18

引用本文的文献

[1]
Influenza-specific antibody-mediated and complement-dependent cellular cytotoxicity-inducing antibodies in vaccinated and infected pigs.

Front Immunol. 2025-6-30

[2]
Targeting Respiratory Viruses: The Efficacy of Intranasal mRNA Vaccination in Generating Protective Mucosal and Systemic Immunity Against Influenza A (H1N1).

Influenza Other Respir Viruses. 2025-3

[3]
Evaluation of mucosal adjuvants to chitosan-nanoparticle-based oral subunit vaccine for controlling salmonellosis in broilers.

Front Immunol. 2025-2-3

[4]
Human Infant Fecal Microbiota Differentially Influences the Mucosal Immune Pathways Upon Influenza Infection in a Humanized Gnotobiotic Pig Model.

Curr Microbiol. 2024-7-14

[5]
Reassortment incompetent live attenuated and replicon influenza vaccines provide improved protection against influenza in piglets.

NPJ Vaccines. 2024-7-13

[6]
Evaluation of Efficacy of Surface Coated versus Encapsulated Influenza Antigens in Mannose-Chitosan Nanoparticle-Based Intranasal Vaccine in Swine.

Vaccines (Basel). 2024-6-11

[7]
Swine influenza A virus: challenges and novel vaccine strategies.

Front Cell Infect Microbiol. 2024

[8]
Does Vaccine-Induced Maternally-Derived Immunity Protect Swine Offspring against Influenza a Viruses? A Systematic Review and Meta-Analysis of Challenge Trials from 1990 to May 2021.

Animals (Basel). 2023-10-3

[9]
Lymph node targeting for immunotherapy.

Immunooncol Technol. 2023-6-28

[10]
An intranasal vaccine comprising SARS-CoV-2 spike receptor-binding domain protein entrapped in mannose-conjugated chitosan nanoparticle provides protection in hamsters.

Sci Rep. 2023-7-26

本文引用的文献

[1]
Chitosan-adjuvanted Salmonella subunit nanoparticle vaccine for poultry delivered through drinking water and feed.

Carbohydr Polym. 2020-9-1

[2]
Immune Response to Enteritidis Infection in Broilers Immunized Orally With Chitosan-Based Subunit Nanoparticle Vaccine.

Front Immunol. 2020

[3]
A Nanoparticle-Poly(I:C) Combination Adjuvant Enhances the Breadth of the Immune Response to Inactivated Influenza Virus Vaccine in Pigs.

Vaccines (Basel). 2020-5-18

[4]
In vitro characterization and immunogenicity of chitosan nanoparticles loaded with native and inactivated extracellular proteins from a field strain of Clostridium perfringens associated with necrotic enteritis.

Vet Immunol Immunopathol. 2020-6

[5]
Intranasally administered protein coated chitosan nanoparticles encapsulating influenza H9N2 HA2 and M2e mRNA molecules elicit protective immunity against avian influenza viruses in chickens.

Vet Res. 2020-3-6

[6]
Poly(I:C) augments inactivated influenza virus-chitosan nanovaccine induced cell mediated immune response in pigs vaccinated intranasally.

Vet Microbiol. 2020-2-13

[7]
Oral Deliverable Mucoadhesive Chitosan- Subunit Nanovaccine for Layer Chickens.

Int J Nanomedicine. 2020-2-3

[8]
Targeted Delivery of Mannosylated Nanoparticles Improve Prophylactic Efficacy of Immersion Vaccine against Fish Viral Disease.

Vaccines (Basel). 2020-2-15

[9]
Mucosal vaccines: Strategies and challenges.

Immunol Lett. 2019-10-25

[10]
Immunogenicity evaluation of rBoNT/E nanovaccine after mucosal administration.

Iran J Basic Med Sci. 2019-4

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索