Intracellular ethanol-mediated oxidation and apoptosis in HepG2/CYP2E1 cells impaired by two active peptides from seahorse ( bleeler) protein hydrolysates via the Nrf2/HO-1 and akt pathways.

Seahorse ( Bleeler) are representative marine species in aquaculture, with special value of medicine and food. In this study, the protective effects of two peptides from seahorse hydrolysates (SHP-1 and SHP-2) against ethanol-mediated oxidative stress in HepG2/CYP2E1 cells were investigated. Firstly, SHP-1 and SHP-2 presented no cytotoxicity. Compared with the ethanol-treated groups, SHP-1 and SHP-2 increased cell viability in a concentration-dependent manner. Secondly, SHP-1 and SHP-2 markedly reduced intracellular reactive oxygen species (ROS) generation, gamma-glutamyltranspeptidase (GGT) activity, and tumor necrosis factor-α (TNF-α) levels and remarkably enhanced superoxide dismutase (SOD) and glutathione (GSH) activities. SHP-1 and SHP-2 also down-regulated the expressions of GGT, bax, c-caspase-8/-9/-3, p-Akt, p-IκB-α, p-p65, p-ERK, and p-p38 but up-regulated SOD, GSH, NF-E2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and bcl-2 levels, as revealed by Western blot analysis. Moreover, SHP-1 and SHP-2 increased the mitochondrial membrane potential (MMP), reduced DNA damage, and suppressed the nuclear translocation of p65. These results suggest that two peptides from seahorse hydrolysates can be considered a potential functional biomaterial and further improve the use value of seahorse in aquaculture.