Petagine Lucy, Zariwala Mohammed G, Somavarapu Satyanarayana, Chan Stefanie Ho Yi, Patel Vinood B
Centre for Nutraceuticals, School of Life Sciences, University of Westminster, London W1W 6UW, UK.
Department of Pharmaceutics, UCL School of Pharmacy, London WC1N 1AX, UK.
Biology (Basel). 2025 Apr 23;14(5):455. doi: 10.3390/biology14050455.
During chronic alcohol misuse, hepatic iron overload occurs, leading to exacerbated oxidative stress and liver injury. The aim was to study formulations encapsulated with the antioxidant curcumin to assess their ability protect against oxidative stress in a model of alcohol-related liver disease (ALD) combined with iron. HepG2 (VL-17A) cells were treated with iron (50 µM) alone or with alcohol (200 to 350 mM) over 72 h and markers of oxidative damage, cell death, and mitochondrial function were assessed. Nanoformulations encapsulating curcumin were also studied. VL-17A cells treated with both ethanol and iron showed significant decreases in cell viability (64%, < 0.0001) when compared to control, and a 56% decrease ( = 0.0279) when compared to iron-only treatment. Iron-alone treatment caused a 115% increase ( < 0.0001) in ROS at 48 h as well as increases of up to 118% when treated with 200 mM ethanol + 50 μM iron ( < 0.0001), compared to control DMEM. The study found that 10 µM curcumin DSPE-PEG increased cell viability by 17% and 41% when compared to control and iron treatment alone, respectively. Formulations reduced ROS by 36% ( = 0.0015) when compared to iron-alone treatment. In summary, encapsulated curcumin provided antioxidant capacity and reduced oxidative stress, demonstrating the therapeutic potential for curcumin formulations in ALD combined with iron dysregulation.
在长期滥用酒精的过程中，肝脏会出现铁过载，导致氧化应激加剧和肝损伤。本研究旨在探讨用抗氧化剂姜黄素包封的制剂，以评估其在酒精性肝病（ALD）合并铁过载模型中对抗氧化应激的能力。将HepG2（VL-17A）细胞单独用铁（50μM）或与酒精（200至350mM）共同处理72小时，然后评估氧化损伤、细胞死亡和线粒体功能的标志物。还研究了包封姜黄素的纳米制剂。与对照组相比，同时用乙醇和铁处理的VL-17A细胞的细胞活力显著降低（64%，<0.0001），与仅用铁处理相比降低了56%（=0.0279）。与对照DMEM相比，仅用铁处理在48小时时导致ROS增加115%（<0.0001），在用200mM乙醇+50μM铁处理时ROS增加高达118%（<0.0001）。研究发现，与对照组和仅用铁处理相比，10μM姜黄素DSPE-PEG分别使细胞活力提高了17%和41%。与仅用铁处理相比，制剂使ROS降低了36%（=0.0015）。总之，包封的姜黄素具有抗氧化能力并降低了氧化应激，证明了姜黄素制剂在ALD合并铁失调中的治疗潜力。
