Zhang Bo, Zhou Tian, Wu Haijing, Zhao Ming, Lu Qianjin
Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China.
Department of Dermatology, Second Xiangya Hospital, Central South University, Hunan Key Laboratory of Medical Epigenomics, Changsha, Hunan, China.
J Transl Autoimmun. 2021 Mar 1;4:100092. doi: 10.1016/j.jtauto.2021.100092. eCollection 2021.
Lupus erythematosus (LE) is an autoimmune disease that can be divided into two types. The cutaneous lupus erythematosus (CLE), such as discoid LE (DLE), affects only the skin. While the systemic lupus erythematosus (SLE) affects the hematopoietic, renal, and other systems. We previously found that methylation could be a biomarker for SLE. Here, we detect the methylation by high-resolution melting-quantitative polymerase chain reaction (HRM-qPCR) assay. The positive percentages of SLE, DLE and healthy controls (HC) are 96.00%, 27.45%, 2.00%, if the curve of 25% methylation was used as the threshold of SLE. And we determined the serum IFN-a1 level by enzyme-linked immunosorbent assay (ELISA) in SLE, DLE and HC. The serum concentration of IFN-a1 in patients with SLE was significantly higher than in the DLE (12.63 ± 6.38 pg/mL vs 7.99 ± 2.28 pg/mL, < 0.05) and HC (12.63 ± 6.38 pg/mL vs 7.17 ± 1.86 pg/mL, < 0.05). But the expression level of IFN-a1 in serum was not significantly different between DLE and HC (7.99 ± 2.28 pg/mL vs 7.17 ± 1.86 pg/mL, P = 0.5365). This suggests that methylation of and serum concentration of IFN-a1 may be used as biomarkers to distinguish DLE from SLE.
红斑狼疮（LE）是一种自身免疫性疾病，可分为两种类型。皮肤型红斑狼疮（CLE），如盘状红斑狼疮（DLE），仅累及皮肤。而系统性红斑狼疮（SLE）则累及造血系统、肾脏及其他系统。我们之前发现甲基化可能是系统性红斑狼疮的一个生物标志物。在此，我们通过高分辨率熔解定量聚合酶链反应（HRM-qPCR）检测甲基化情况。若将25%甲基化曲线作为系统性红斑狼疮的阈值，则系统性红斑狼疮、盘状红斑狼疮和健康对照（HC）的阳性率分别为96.00%、27.45%、2.00%。并且我们通过酶联免疫吸附测定（ELISA）测定了系统性红斑狼疮、盘状红斑狼疮和健康对照者血清中的IFN-α1水平。系统性红斑狼疮患者血清中IFN-α1的浓度显著高于盘状红斑狼疮患者（12.63 ± 6.38 pg/mL对7.99 ± 2.28 pg/mL，<0.05）和健康对照者（12.63 ± 6.38 pg/mL对7.17 ± 1.86 pg/mL，<0.05）。但盘状红斑狼疮患者与健康对照者血清中IFN-α1的表达水平无显著差异（7.99 ± 2.28 pg/mL对7.17 ± 1.86 pg/mL，P = 0.5365）。这表明[此处原文可能缺失相关基因名称]的甲基化和血清IFN-α1浓度可能用作区分盘状红斑狼疮和系统性红斑狼疮的生物标志物。
