皮肤狼疮中的 microRNA 表达：理解其发病机制的新窗口。
MicroRNA Expression in Cutaneous Lupus: A New Window to Understand Its Pathogenesis.
机构信息
Department of Dermatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico.
Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico.
出版信息
Mediators Inflamm. 2019 Dec 30;2019:5049245. doi: 10.1155/2019/5049245. eCollection 2019.
BACKGROUND
The role of miRNAs in the pathogenesis of cutaneous lupus has not been studied.
OBJECTIVE
It was to assess the levels of a selected panel of circulating miRNAs that could be involved in the regulation of the immune response, inflammation, and fibrosis in cutaneous lupus.
METHODS
It was a cross-sectional study. We included 22 patients with subacute (SCLE) and 20 with discoid (DLE) lesions, and 19 healthy donors (HD). qRT-PCR for miRNA analysis, flow cytometry in peripheral blood, and skin immunohistochemistry were performed to determine the distribution of CD4 T cells and regulatory cells and their correlation with circulating miRNAs.
RESULTS
miR-150, miR-1246, miR-21, miR-23b, and miR-146 levels were downregulated in SCLE HD. miR-150, miR-1246, and miR-21 levels were downregulated in DLE HD. miR-150, miR-1246, and miR-21 levels were downregulated in DLE with miR-1246 in SCLE, whereas CD123/CD196/IDO cells were positively associated with miR-150 in DLE. In the tissue, CD4/IL-4 and CD20/IL-10 cells were positively associated with miR-21 and CD4/IFN- with miR-1246 in SCLE, whereas CD123/CD196/IDO cells were positively associated with miR-150 in DLE. In the tissue, CD4/IL-4 and CD20/IL-10 cells were positively associated with miR-21 and CD4/IFN-, thyroid hormone, and cancer signaling pathways were shared between miR-21, miR-31, miR-23b, miR-146a, miR-1246, and miR-150.
CONCLUSIONS
A downregulation of miR-150, miR-1246, and miR-21 in both CLE varieties HD. miR-150, miR-1246, and miR-21 levels were downregulated in DLE.
背景
miRNAs 在皮肤狼疮发病机制中的作用尚未得到研究。
目的
评估一组可能参与调节皮肤狼疮免疫反应、炎症和纤维化的循环 miRNA 的水平。
方法
这是一项横断面研究。我们纳入了 22 例亚急性（SCLE）和 20 例盘状（DLE）病变患者，以及 19 名健康供体（HD）。进行 miRNA 分析的 qRT-PCR、外周血流式细胞术和皮肤免疫组织化学检测，以确定 CD4 T 细胞和调节性细胞的分布及其与循环 miRNA 的相关性。
结果
miR-150、miR-1246、miR-21、miR-23b 和 miR-146 在 SCLE 与 HD 之间的水平下调。miR-150、miR-1246 和 miR-21 在 DLE 与 HD 之间的水平下调。miR-150、miR-1246 和 miR-21 在 DLE 与 HD 之间的水平下调，而 miR-1246 在 SCLE 中与 CD123/CD196/IDO 细胞呈正相关，而 CD4/IL-4 和 CD20/IL-10 细胞在 DLE 中与 miR-21 呈正相关。在组织中，CD4/IL-4 和 CD20/IL-10 细胞与 miR-21 呈正相关，而 CD4/IFN-γ 与 miR-1246 在 SCLE 中呈正相关，而 CD123/CD196/IDO 细胞在 DLE 中与 miR-150 呈正相关。在组织中，CD4/IL-4 和 CD20/IL-10 细胞与 miR-21 呈正相关，而 CD4/IFN-γ 与 miR-1246 在 SCLE 中呈正相关，而 CD123/CD196/IDO 细胞在 DLE 中与 miR-150 呈正相关。甲状腺激素和癌症信号通路在 miR-21、miR-31、miR-23b、miR-146a、miR-1246 和 miR-150 之间存在相关性。
结论
miR-150、miR-1246 和 miR-21 在两种 CLE 类型中均下调，与 HD 相比。miR-150、miR-1246 和 miR-21 在 DLE 中下调。
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