Université Grenoble Alpes, Inserm, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 38000 Grenoble, France.
STAR Protoc. 2021 Mar 8;2(1):100382. doi: 10.1016/j.xpro.2021.100382. eCollection 2021 Mar 19.
BDNF levels are reduced in the chronically stressed brain, in the area of hippocampus. Part of the hippocampal BDNF is provided by neuronal projection of the entorhinal cortex. Studying the cortico-hippocampal transport of BDNF is technically difficult. Here, we describe a protocol that reproduces mouse cortico-hippocampal circuit by plating neurons on the microfluidic devices and infecting the neurons with virus-encoding BDNF-mCherry, which allows investigation of the effects of elevated corticosterone levels on BDNF axonal transport. For complete details on the use and execution of this protocol, please refer to Agasse et al. (2020).
BDNF 水平在慢性应激大脑中降低，特别是在海马体区域。海马体的部分 BDNF 是由内嗅皮层的神经元投射提供的。研究 BDNF 的皮质-海马转运在技术上具有一定难度。在这里，我们描述了一个方案，通过在微流控设备上种植神经元并感染携带 BDNF-mCherry 的病毒来复制小鼠皮质-海马回路，这允许研究升高的皮质酮水平对 BDNF 轴突运输的影响。有关此方案使用和执行的完整详细信息，请参阅 Agasse 等人（2020 年）。
