Faculty of Biotechnology, Jeju National University, Jeju, 63243, Republic of Korea.
Department of Biological Sciences and Biotechnology, Hannam University, Daejeon, 34054, Republic of Korea.
Arch Microbiol. 2021 Aug;203(6):2843-2852. doi: 10.1007/s00203-021-02191-z. Epub 2021 Mar 22.
A Gram-reaction-negative, strictly aerobic, betaproteobacterial strain, designated SAP-35, was isolated from sap extracted from Acer pictum in Mt. Halla in Jeju, Republic of Korea, and its taxonomic status was examined by a polyphasic approach. Cells of the organism were non-sporulating, motile rods and grew at 4-30 °C, pH 6-7 and in the absence of NaCl. 16S rRNA gene- and whole genome-based phylogenetic analyses showed that strain SAP-35 belonged to the family Oxalobacteraceae and was closely related to Rugamonas rivuli (98.9% 16S rRNA gene sequence similarity) and Rugamonas aquatica (98.4%). The phylogenomic clustering and average amino acid identity values supported that strain SAP-35 belonged to the genus Duganella and two Rugamonas species should be transferred to the genus Duganella. The major isoprenoid quinone of the isolate was Q-8. The major polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospholipid. The predominant fatty acids were summed feature 3, C and C cyclo. The G + C content of genome was 64.9%. The average nucleotide identity and dDDH values between strain SAP-35 and the members of the genera Rugamonas and Duganella were < 85.1% and < 49%, respectively. Based on the combined data presented here, strain SAP-35 (= KCTC 72227 = NBRC 113903) represents a novel species of the genus Duganella, for which the name Duganella aceris sp. nov. is proposed. Also, Rugamonas aquatica Lu et al. (Int J Syst Evol Microbiol 70: 3328-3334, 2020) and Rugamonas aquatica Lu et al. 2020 are reclassified as Duganella aquatica comb. nov., with the emended description of the genus Rugamonas.
一株革兰氏反应阴性、严格需氧、β变形杆菌菌株，被命名为 SAP-35，从韩国济州岛汉拿山的 Acer pictum 树液中分离得到，并通过多相研究方法对其分类地位进行了研究。该菌细胞为非芽孢形成、运动杆状，在 4-30°C、pH 值 6-7 和无 NaCl 的条件下生长。16S rRNA 基因和全基因组系统发育分析表明，菌株 SAP-35 属于 Oxalobacteraceae 科，与 Rugamonas rivuli（98.9% 16S rRNA 基因序列相似性）和 Rugamonas aquatica（98.4%）最为密切相关。系统发育聚类和平均氨基酸同一性值支持菌株 SAP-35 属于 Duganella 属，两个 Rugamonas 种应转移到 Duganella 属。分离株的主要类异戊二烯醌为 Q-8。主要极性脂类为磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油和一种未鉴定的氨基磷脂。主要脂肪酸为总和特征 3、C 和 C 环。基因组的 G+C 含量为 64.9%。菌株 SAP-35 与 Rugamonas 和 Duganella 属成员之间的平均核苷酸同一性和 dDDH 值均<85.1%和<49%。基于这里提出的综合数据，菌株 SAP-35（=KCTC 72227=NRBC 113903）代表 Duganella 属的一个新种，命名为 Aceris 杜根氏菌。同时，Rugamonas aquatica Lu 等人（Int J Syst Evol Microbiol 70: 3328-3334, 2020）和 Rugamonas aquatica Lu 等人 2020 年被重新分类为 Duganella aquatica comb. nov.，对 Rugamonas 属的描述进行了修订。
