聚乙二醇介导的疫苗颗粒组装以提高稳定性和免疫原性。
Poly(ethylene glycol)-Mediated Assembly of Vaccine Particles to Improve Stability and Immunogenicity.
机构信息
Key Laboratory of Colloid and Interface Chemistry of the Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, China.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, China.
出版信息
ACS Appl Mater Interfaces. 2021 Mar 31;13(12):13978-13989. doi: 10.1021/acsami.1c00706. Epub 2021 Mar 22.
We report the one-step assembly of vaccine particles by encapsulating ovalbumin (OVA) and cytosine-phosphate-guanine oligodeoxynucleotides (CpG) into poly(ethylene glycol) (PEG)-mediated zeolitic imidazolate framework-8 nanoparticles (OVA-CpG@ZIF-8 NPs), where PEG improves the stability and dispersity of ZIF-8 NPs and the NPs protect the encapsulated OVA and CpG to circumvent the cold chain issue. Compared with free OVA and OVA-encapsulated ZIF-8 (OVA@ZIF-8) NPs, OVA-CpG@ZIF-8 NPs can enhance antigen uptake, cross-presentation, dendritic cell (DC) maturation, production of specific antibody and cytokines, and CD4 T and CD8 T cell activation. More importantly, the vaccine particles retain their bioactivity against enzymatic degradation, elevated temperatures, and long-term storage at ambient temperature. The study highlights the importance of PEG-mediated ZIF-8 NPs as a vaccine delivery system for the promising application of effective and cold chain-independent vaccination against diseases.
我们报告了通过将卵清蛋白（OVA）和胞嘧啶-磷酸-鸟嘌呤寡脱氧核苷酸（CpG）包封到聚乙二醇（PEG）介导的沸石咪唑酯骨架-8 纳米颗粒（OVA-CpG@ZIF-8 NPs）中来一步组装疫苗颗粒，其中 PEG 提高了 ZIF-8 NPs 的稳定性和分散性，并且 NPs 保护了包封的 OVA 和 CpG，以规避冷链问题。与游离 OVA 和包封 OVA 的 ZIF-8（OVA@ZIF-8）纳米颗粒相比，OVA-CpG@ZIF-8 NPs 可以增强抗原摄取、交叉呈递、树突状细胞（DC）成熟、特异性抗体和细胞因子的产生以及 CD4 T 和 CD8 T 细胞的激活。更重要的是，疫苗颗粒在对抗酶降解、高温和长期在环境温度下储存时保持其生物活性。该研究强调了 PEG 介导的 ZIF-8 NPs 作为疫苗递送系统的重要性，为针对疾病的有效且无需冷链的疫苗接种提供了有前途的应用。
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