ARC Centre of Excellence for Electromaterials Science (ACES), Intelligent Polymer Research Institute, University of Wollongong, Wollongong, Australia.
Department of Bioengineering, The University of Texas at Dallas, Richardson, Texas 75080, USA.
Biointerphases. 2021 Mar 22;16(2):021003. doi: 10.1116/6.0000708.
We present the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) doped with an algal-derived glycan extract, Phycotrix™ [xylorhamno-uronic glycan (XRU84)], as an innovative electrically conductive material capable of providing beneficial biological and electrical cues for the promotion of favorable wound healing processes. Increased loading of the algal XRU84 into PEDOT resulted in a reduced surface nanoroughness and interfacial surface area and an increased static water contact angle. PEDOT-XRU84 films demonstrated good electrical stability and charge storage capacity and a reduced impedance relative to the control gold electrode. A quartz crystal microbalance with dissipation monitoring study of protein adsorption (transferrin, fibrinogen, and collagen) showed that collagen adsorption increased significantly with increased XRU84 loading, while transferrin adsorption was significantly reduced. The viscoelastic properties of adsorbed protein, characterized using the ΔD/Δf ratio, showed that for transferrin and fibrinogen, a rigid, dehydrated layer was formed at low XRU84 loadings. Cell studies using human dermal fibroblasts demonstrated excellent cell viability, with fluorescent staining of the cell cytoskeleton illustrating all polymers to present excellent cell adhesion and spreading after 24 h.
我们提出了一种导电聚合物聚（3,4-乙二氧基噻吩）（PEDOT），其中掺杂了一种藻类衍生的聚糖提取物 Phycotrix [木糖醛酸-鼠李糖聚糖（XRU84）]，作为一种创新的导电材料，能够为促进有利的伤口愈合过程提供有益的生物学和电学线索。将藻类 XRU84 更多地负载到 PEDOT 中，会导致表面纳米粗糙度降低、界面表面积增加和静态水接触角增加。PEDOT-XRU84 薄膜具有良好的电稳定性和电荷存储能力，并且与对照金电极相比，阻抗降低。石英晶体微天平耗散监测研究表明，蛋白质（转铁蛋白、纤维蛋白原和胶原蛋白）的吸附量随着 XRU84 负载量的增加而显著增加，而转铁蛋白的吸附量则显著减少。使用ΔD/Δf 比表征吸附蛋白质的粘弹性性质表明，对于转铁蛋白和纤维蛋白原，在低 XRU84 负载量下形成了刚性、脱水层。使用人真皮成纤维细胞进行的细胞研究表明，细胞活力极好，细胞骨架的荧光染色表明所有聚合物在 24 小时后均具有极好的细胞黏附和铺展。
