Yang Junyan, Kim Dong Hwan, Hendricks Jeffrey L, Leach Michelle, Northey Rebecca, Martin David C
Department of Materials Science and Engineering, 2022 H. H. Dow Building, The University of Michigan, Ann Arbor, MI 48109-2136, USA.
Acta Biomater. 2005 Jan;1(1):125-36. doi: 10.1016/j.actbio.2004.09.006.
Ordered conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) was electrochemically fabricated using a self-assembled medium of surfactant molecules as a template. The morphology and microstructure were extensively investigated by optical and electron microscopy, and results show that the coated films were composed of anisotropic domains having a characteristic size of 15-150 nm. The surfactant-templated ordered PEDOT films were electrochemically deposited on microfabricated neural probes with an electrode site area of 1,256 microm(2). The electrical properties were studied by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). EIS showed a lowered magnitude of 35 kOmega (from an initial approximately 800 kOmega) at the biologically relevant frequency of 1 kHz. CV results show that the film has higher charge capacity and is more electrochemically stable than either nodular PEDOT or PPy. Furthermore, we have begun to probe the biological response to such a material intended to define the tissue-material interface. Results show that minute concentrations of the non-ionic surfactant are enough to kill all nearby cells in culture. It is possible however, to create surfactant-templated ordered PEDOT such that SH-SY5Y survive on the conductive polymer.
采用表面活性剂分子自组装介质作为模板，通过电化学方法制备了有序导电聚合物聚（3,4 - 亚乙基二氧噻吩）（PEDOT）。利用光学显微镜和电子显微镜对其形态和微观结构进行了广泛研究，结果表明，涂层薄膜由特征尺寸为15 - 150 nm的各向异性域组成。将表面活性剂模板化的有序PEDOT薄膜电化学沉积在电极位点面积为1256平方微米的微加工神经探针上。通过电化学阻抗谱（EIS）和循环伏安法（CV）研究了其电学性质。EIS显示，在1 kHz的生物相关频率下，其电阻值降低至35 kΩ（初始值约为800 kΩ）。CV结果表明，该薄膜具有更高的电荷容量，并且比结节状PEDOT或聚吡咯（PPy）在电化学上更稳定。此外，我们已开始探究这种材料的生物学反应，以确定组织 - 材料界面。结果表明，微量的非离子表面活性剂足以杀死培养物中所有附近的细胞。然而，有可能制备出表面活性剂模板化的有序PEDOT，使SH - SY5Y细胞在导电聚合物上存活。
