Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.
School of medicine, northwest minzu university, Lanzhou, China.
BMC Microbiol. 2021 Mar 22;21(1):87. doi: 10.1186/s12866-021-02150-0.
Minqin is suffering from a serious desertification, whereas the knowledge about its bacterial community is limited. Herein, based on Nitraria tangutorum and Haloxylon ammodendron from Minqin, the bacterial community diversities in fixed sandy land, semi-fixed sandy land and shifting sandy land were investigated by combining with culture-dependent and culture-independent methods.
Minqin stressed with high salinity and poor nutrition is an oligotrophic environment. Bacterial community in Minqin was shaped primarily by the presence of host plants, whereas the type of plant and sandy land had no marked effect on those, which displayed a better survival in the rhizospheres of N. tangutorum and H. ammodendron. The dominant groups at phyla level were Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, Planctomycetes, Chloroflexi, Acidobacteria and Candidate_division_TM7. The abundance of Firmicutes with ability of desiccation-tolerance was significantly higher in harsh environment, whereas Bacteroidetes were mainly distributed in areas with high nutrient content. The abundances of Proteobacteria and Bacteroidetes were relatively high in the rhizospheres of N. tangutorum and H. ammodendron, which had more plant-growth promoting rhizobacteria. A large number of Actinobacteria were detected, of which the most abundant genus was Streptomyces. The physicochemical factors related to the diversity and distribution of the bacterial community were comprehensively analyzed, such as pH, electrical conductivity, soil organic matter, C/N and sand, and the results indicated that Minqin was more suitable for the growth of N. tangutorum, which should be one of most important sand-fixing plants in Minqin.
The bacterial community diversities in different types of sandy lands of Minqin were comprehensively and systematically investigated by culture-dependent and culture-independent approaches, which has a great significance in maintaining/restoring biological diversity.
民勤遭受严重的沙漠化，但其细菌群落知识有限。本研究以民勤的白刺和梭梭为对象，结合培养依赖和非培养依赖方法，调查了固定沙丘、半固定沙丘和流动沙丘中细菌群落的多样性。
民勤是一个高盐度和营养贫瘠的贫营养环境，细菌群落主要由宿主植物决定，而植物类型和沙丘类型对其没有明显影响，它们在白刺和梭梭的根际中表现出更好的生存能力。在门水平上的主要类群是放线菌、厚壁菌门、变形菌门、拟杆菌门、浮霉菌门、绿弯菌门、酸杆菌门和候选门 TM7。具有干燥耐性能力的厚壁菌门的丰度在恶劣环境中显著较高，而拟杆菌门主要分布在高营养含量的区域。在白刺和梭梭的根际中，变形菌门和拟杆菌门的丰度相对较高，它们具有更多的植物促生根际细菌。检测到大量的放线菌，其中最丰富的属是链霉菌属。综合分析了与细菌群落多样性和分布相关的理化因素，如 pH 值、电导率、土壤有机质、C/N 和沙，结果表明，民勤更适合白刺生长，白刺应该是民勤最重要的固沙植物之一。
本研究采用培养依赖和非培养依赖方法，综合系统地调查了民勤不同类型沙丘中的细菌群落多样性，对维护/恢复生物多样性具有重要意义。
