Department of Applied Biology, Adama Science and Technology University, Adama, Oromia, Ethiopia.
Department of Applied Chemistry, Adama Science and Technology University, Adama, Oromia, Ethiopia.
Curr Microbiol. 2023 Nov 6;80(12):405. doi: 10.1007/s00284-023-03513-8.
Medicinal plants are known to harbor diverse species of endophytic bacteria which are known for secretion of beneficial secondary metabolites, like enzymes and antimicrobial compounds. The present study aimed to isolate, characterize, and identify the endophytic bacteria isolates from Artemisia annua, Moringa oleifera, and Ocimum lamiifolium plants. Certain endophytic bacterial isolates were screened. Phosphate and Zinc solubilization were performed for newly obtained isolates. The 16S rRNA gene sequencing was performed for RPAAI-8 isolate. Data were analyzed. Our study showed that endophytic bacterial isolates were recognized to be Bacillus cereus, B. subtilis, Citrobacter freundii, Enterobacter asburiae, E. cloacae, E. kobei, E. ludwigii, Enterococcus faecium, and Pseudomonas monteilli. From among these differentiated endophytic bacterial isolates, Enterobacter species are the most frequently obtained isolates. These bacterial isolates were shown 99.77% sequence similarity to Enterobacter ludwigii EN-119 (JTLO01000001) using 16S rRNA gene sequencing. This isolate was designated as Enterobacter sp. RPAAI-8. This isolate was able to employ selected cheap and cost-effective agro wastes as a carbon source. This cheap agro waste utilization by these Enterobacter species could be the first report. In conclusion, the present isolates are found to be employed for plant growth promotion and solubilizing insoluble phosphate and zinc. Before this time, most of the recent isolates were not identified from these medicinal plants. The ethyl acetate extract of the isolates also showed inhibitory activity against selected test pathogens.
药用植物被认为蕴藏着多种内生细菌，这些内生细菌以分泌有益的次生代谢产物而闻名，如酶和抗菌化合物。本研究旨在从青蒿、辣木和罗勒植物中分离、鉴定内生细菌。对某些内生细菌进行了筛选。对新获得的分离株进行了磷酸盐和锌的溶解。对 RPAAI-8 分离株进行了 16S rRNA 基因测序。数据分析。我们的研究表明，内生细菌分离株被鉴定为蜡样芽孢杆菌、枯草芽孢杆菌、弗氏柠檬酸杆菌、阿氏肠杆菌、阴沟肠杆菌、凯氏肠杆菌、鲁氏肠杆菌、屎肠球菌和蒙氏假单胞菌。在这些不同的内生细菌分离株中，肠杆菌属是最常获得的分离株。这些细菌分离株与 16S rRNA 基因测序的鲁氏肠杆菌 EN-119（JTLO01000001）具有 99.77%的序列相似性。该分离株被命名为肠杆菌属 RPAAI-8。该分离株能够利用选定的廉价和具有成本效益的农业废弃物作为碳源。肠杆菌属利用这些廉价的农业废弃物可能是首次报道。总之，本研究发现这些分离株可用于促进植物生长、溶解不溶性磷酸盐和锌。在此之前，最近的大多数分离株都没有从这些药用植物中鉴定出来。分离株的乙酸乙酯提取物也显示出对选定的测试病原体的抑制活性。
