Young Researchers and Elite Club, Saveh Branch, Islamic Azad University, Saveh, Iran.
J Biosci. 2021;46.
Due to the broad-spectrum of antibiotic resistance, herein we investigated the possibility of using imipenemconjugated silver nanoparticles (IMP-AgNPs) against multidrug-resistant isolates of . For this purpose, 200 clinical isolates were tested against different antibiotics to determine the antimicrobial susceptibility. To identify and resistance genes, PCR was used. The synthesized AgNPs and conjugants were characterized using UV-vis spectroscopy, XRD, SEM, TEM, DLS, and FTIR. The stability, drug release kinetics, cytotoxicity, hemolytic and apoptotic effects of NPs were also investigated. MIC of the imipenem, AgNPs, and conjugants were evaluated versus isolates. Finally, the effects of the IMP-AgNPs to heal burn wounds in rats was evaluated. According to the results, about 68% of isolates showed resistance to imipenem (MIC ≥ 64 μg/ml to ≥ 512 μg/ml). Analytical results verified the synthesis of AgNPs and IMP-AgNPs. A Dose-dependent decrease happened in terms of the MIC values of IMP-AgNPs were also affected by the existence of resistant genes. Low cytotoxic was observed regarding AgNPs which lead to apoptosis. The histopathological results showed a considerable epithelization in treated groups with IMPAgNPs. Accordingly, IMP-AgNPs can be considered as a powerful antibacterial agent to treat the infections caused by multidrug-resistant .
由于抗生素耐药性广泛，我们在此研究了使用碳青霉烯类抗生素结合银纳米粒子（IMP-AgNPs）来对抗多药耐药株的可能性。为此，我们对 200 株临床分离株进行了不同抗生素的药敏试验，以确定其抗菌敏感性。为了鉴定 和 耐药基因，我们使用 PCR 进行了检测。我们使用紫外可见光谱、XRD、SEM、TEM、DLS 和 FTIR 对合成的 AgNPs 和缀合物进行了表征。还研究了 NPs 的稳定性、药物释放动力学、细胞毒性、溶血和凋亡作用。我们评估了碳青霉烯类抗生素、AgNPs 和缀合物对 分离株的 MIC。最后，我们还评估了 IMP-AgNPs 对大鼠烧伤创面愈合的影响。结果表明，约 68%的分离株对亚胺培南（MIC 值为 64μg/ml 至≥512μg/ml）耐药。分析结果证实了 AgNPs 和 IMP-AgNPs 的合成。IMP-AgNPs 的 MIC 值也呈剂量依赖性下降，而且耐药基因的存在也会影响其下降程度。AgNPs 的细胞毒性较低，导致细胞凋亡。组织病理学结果显示，IMP-AgNPs 治疗组有明显的上皮化。因此，IMP-AgNPs 可以被认为是一种有效的抗菌药物，用于治疗多药耐药株引起的感染。
