密歇根家庭流感疫苗评估（HIVE）队列中冠状病毒的发生：再感染频率和对季节性和严重急性呼吸综合征冠状病毒的血清学反应。

Coronavirus Occurrence in the Household Influenza Vaccine Evaluation (HIVE) Cohort of Michigan Households: Reinfection Frequency and Serologic Responses to Seasonal and Severe Acute Respiratory Syndrome Coronaviruses.

机构信息

Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, Michigan, USA.

National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA.

出版信息

J Infect Dis. 2021 Jul 2;224(1):49-59. doi: 10.1093/infdis/jiab161.

DOI:10.1093/infdis/jiab161

PMID:33755731

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8083771/

Abstract

BACKGROUND

We investigated frequency of reinfection with seasonal human coronaviruses (HCoVs) and serum antibody response following infection over 8 years in the Household Influenza Vaccine Evaluation (HIVE) cohort.

METHODS

Households were followed annually for identification of acute respiratory illness with reverse-transcription polymerase chain reaction-confirmed HCoV infection. Serum collected before and at 2 time points postinfection were tested using a multiplex binding assay to quantify antibody to seasonal, severe acute respiratory syndrome coronavirus (SARS-CoV), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike proteins and SARS-CoV-2 spike subdomains and N protein.

RESULTS

Of 3418 participants, 40% were followed for ≥3 years. A total of 1004 HCoV infections were documented; 303 (30%) were reinfections of any HCoV type. The number of HCoV infections ranged from 1 to 13 per individual. The mean time to reinfection with the same type was estimated at 983 days for 229E, 578 days for HKU1, 615 days for OC43, and 711 days for NL63. Binding antibody levels to seasonal HCoVs were high, with little increase postinfection, and were maintained over time. Homologous, preinfection antibody levels did not significantly correlate with odds of infection, and there was little cross-response to SARS-CoV-2 proteins.

CONCLUSIONS

Reinfection with seasonal HCoVs is frequent. Binding anti-spike protein antibodies do not correlate with protection from seasonal HCoV infection.

摘要

背景

我们调查了季节性人类冠状病毒（HCoV）再感染的频率以及在 Household Influenza Vaccine Evaluation（HIVE）队列中感染后 8 年的血清抗体反应。

方法

每年对家庭进行随访，以通过逆转录聚合酶链反应（RT-PCR）确认 HCoV 感染来识别急性呼吸道疾病。在感染前和感染后 2 个时间点采集血清，使用多重结合测定法检测针对季节性、严重急性呼吸综合征冠状病毒（SARS-CoV）和严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）刺突蛋白以及 SARS-CoV-2 刺突亚结构域和 N 蛋白的抗体。

结果

在 3418 名参与者中，40%的参与者随访时间≥3 年。共记录了 1004 例 HCoV 感染；303 例（30%）为任何 HCoV 型别的再感染。个体感染 HCoV 的数量范围为 1 至 13 次。对于 229E、HKU1、OC43 和 NL63，同型再感染的平均时间估计分别为 983 天、578 天、615 天和 711 天。对季节性 HCoV 的结合抗体水平较高，感染后略有增加，并随时间保持不变。同种、感染前的抗体水平与感染的几率没有显著相关性，并且对 SARS-CoV-2 蛋白的交叉反应很小。