Department of Stomatology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao 266003, China; School of Stomatology, Qingdao University, Qingdao 266003, China.
School of Stomatology, Qingdao University, Qingdao 266003, China.
Int J Biol Macromol. 2021 Jun 1;180:599-607. doi: 10.1016/j.ijbiomac.2021.03.065. Epub 2021 Mar 20.
Fucoidan has received much attention in healthy food and biomedicine owing to their unique (bio)physicochemical properties, particularly antibacterial and antiviral. Pathogenic microorganisms and probiotics are coexisting in many tissues (e.g., gut, oral, and vagina). However, the effect of fucoidan on probiotics has not been examined. Herein, fucoidan sterilized by different methods (i.e., 0.22 μm filter and high-temperature autoclave) is applied to explore its effect on the responses of Lactobacillus rhamnosus. It is found that high-temperature autoclave treatment causes the depolymerization of fucoidan. Further, the proliferation, morphology, and metabolism of probiotics are greatly dependent on the concentrations of fucoidan. The formation of probiotic biofilm is reduced with an increased concentration of fucoidan. Moreover, the antibacterial ability of probiotics initially increases and then decreases with an increased concentration of fucoidan. Thus, fucoidan could serve as a new marine-origin prebiotic, offering new insight into probiotic modulation and its application in inhibiting bacterial infections.
由于其独特的（生物）物理化学特性，尤其是抗菌和抗病毒特性，褐藻糖胶在保健食品和生物医学领域受到了广泛关注。病原菌和益生菌共存于许多组织（如肠道、口腔和阴道）中。然而，褐藻糖胶对益生菌的影响尚未得到检验。在此，采用不同方法（即 0.22μm 过滤器和高温高压灭菌器）对褐藻糖胶进行灭菌处理，以探讨其对鼠李糖乳杆菌反应的影响。研究发现，高温高压灭菌处理会导致褐藻糖胶的解聚。此外，益生菌的增殖、形态和代谢对褐藻糖胶的浓度有很大的依赖性。随着褐藻糖胶浓度的增加，益生菌生物膜的形成减少。而且，益生菌的抗菌能力随着褐藻糖胶浓度的增加先增加后减少。因此，褐藻糖胶可以作为一种新型的海洋来源的益生元，为益生菌的调节及其在抑制细菌感染中的应用提供了新的见解。
