Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou 510642, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Phytopathology. 2021 Nov;111(11):2088-2099. doi: 10.1094/PHYTO-11-20-0501-R. Epub 2021 Nov 11.
is ubiquitous in diverse environmental habitats. It merits significant concern because of its increasing incidence of nosocomial and community-acquired infection in immunocompromised patients and multiple drug resistance. It is rarely reported as a phytopathogen except in causing white stripe disease of rice in India and postharvest fruit rot of Lanzhou lily. For this study, and strains were simultaneously isolated from soft rot leaves of in Guangzhou, China, and were both demonstrated to be pathogenic to the host. Compared with the strains, strains propagated faster for greater growth in lysogeny broth medium and produced no cellulases or polygalacturonases, but did produce more proteases and fewer extracellular polysaccharides. Furthermore, strains swam and swarmed dramatically less on semisolid media, but formed a great many more biofilms. Both and strains isolated from clivia caused rot symptoms on other monocot hosts, but not on dicots. Similar to previously reported strains isolated from other sources, the strain JZL8 survived under many antibiotic stresses. The complete genome sequence of strain JZL8 consists of a chromosome of 4,635,432 bp without a plasmid. Pan-genome analysis of JZL8 and 180 other strains identified 50 genes that are unique to JZL8, seven of which implicate JZL8 as the potential pathogen contributor in plants. JZL8 also contains three copies of Type I Secretion System machinery; this is likely responsible for its greater production of proteases. Findings from this study extend our knowledge on the host range of and provide insight into the phenotypic and genetic features underlying the plant pathogenicity of JZL8.
在中国广州，从软腐病百合叶片中同时分离到 和 菌株，均被证明对宿主具有致病性。与 菌株相比， 菌株在溶菌肉汤培养基中繁殖更快，生长更大，不产生纤维素酶或聚半乳糖醛酸酶，但产生更多的蛋白酶和更少的胞外多糖。此外， 菌株在半固体培养基上的泳动和扩散能力显著降低，但形成了更多的生物膜。从朱顶红中分离到的 和 菌株均可在其他单子叶植物宿主上引起腐烂症状，但不能在双子叶植物上引起腐烂症状。与之前从其他来源报道的 菌株相似，JZL8 菌株在许多抗生素压力下存活。JZL8 菌株的全基因组序列由一个 4635432bp 的染色体组成，不含质粒。对 JZL8 和 180 株其他 菌株的泛基因组分析鉴定出 50 个 JZL8 特有的基因，其中 7 个暗示 JZL8 可能是植物病原体的潜在贡献者。JZL8 还含有三个拷贝的 I 型分泌系统机制；这可能是其产生更多蛋白酶的原因。本研究的结果扩展了我们对 宿主范围的认识，并深入了解了 JZL8 植物致病性的表型和遗传特征。
