IDEXX SediVue Dx 尿液沉渣分析仪与手工显微镜检查在犬尿液中检测管型的比较。
Comparison of IDEXX SediVue Dx urine sediment analyzer to manual microscopy for detection of casts in canine urine.
机构信息
Department of Pathology, Microbiology, and Immunology, University of California, School of Veterinary Medicine, Davis, California, USA.
Department of Medicine and Epidemiology, University of California, School of Veterinary Medicine, Davis, California, USA.
出版信息
J Vet Intern Med. 2021 May;35(3):1439-1447. doi: 10.1111/jvim.16090. Epub 2021 Mar 24.
BACKGROUND
Detection of urinary casts is difficult due to their intermittent presence and deterioration in urine samples.
OBJECTIVE
To compare the performance of the IDEXX SediVue Dx Urine Sediment Analyzer (SediVue) with manual microscopy for the detection of urinary casts. We hypothesized that the SediVue analyzer would perform similarly to manual microscopy in cast detection.
ANIMALS
Four hundred forty-three samples from 420 dogs from a hospital population.
METHODS
This is a prospective, cross-sectional study. For SediVue analysis (software version [SW] 1.0.1.3), uncentrifuged urine was pipetted into a disposable cartridge. Seventy images were captured and processed by an onboard algorithm. For manual microscopy, urine was centrifuged to obtain sediment. Any cast identified by either method was considered a positive result (>0/low-power field [LPF]). SediVue images were evaluated if casts were detected by either methodology. A revised sensitivity and specificity were calculated after image review and when using a threshold of >1 cast/LPF.
RESULTS
The sensitivity of the SediVue analysis for the detection of urinary casts was 53.7% (43.85%-63.35%), and specificity was 86.0% (81.78%-89.51%). After image review, the revised sensitivity/specificity was 52.0% (42.89%-61.02%) and 90.6% (86.81%-93.54%), respectively. When using a more clinically relevant threshold of >1/LPF, the sensitivity was 52.6% (35.82%-69.02%) and specificity was 99.3% (97.85%-99.85%).
CONCLUSIONS AND CLINICAL IMPORTANCE
The SediVue provides moderate agreement to manual methodology for detection of casts in urine.
背景
由于尿沉渣间歇性存在且尿液样本质量下降，因此检测尿沉渣较为困难。
目的
比较 IDEXX SediVue Dx 尿液沉渣分析仪（SediVue）与人工显微镜检测尿液沉渣的性能。我们假设 SediVue 分析仪在检测尿沉渣方面的表现与人工显微镜相似。
动物
来自医院人群的 420 只狗的 443 个样本。
方法
这是一项前瞻性、横断面研究。对于 SediVue 分析（软件版本 [SW] 1.0.1.3），未离心的尿液被吸入一次性小瓶中。采集 70 张图像，并由内置算法进行处理。对于人工显微镜，尿液离心获得沉淀物。任何通过两种方法之一识别的管型都被认为是阳性结果（>0/低倍视野 [LPF]）。如果通过任何一种方法检测到管型，则评估 SediVue 图像。在进行图像评估后，以及当使用 >1 管型/LPF 的阈值时，计算重新计算的灵敏度和特异性。
结果
SediVue 分析检测尿沉渣的灵敏度为 53.7%（43.85%-63.35%），特异性为 86.0%（81.78%-89.51%）。图像评估后，重新计算的灵敏度/特异性分别为 52.0%（42.89%-61.02%）和 90.6%（86.81%-93.54%）。当使用更具临床相关性的阈值>1/LPF 时，灵敏度为 52.6%（35.82%-69.02%），特异性为 99.3%（97.85%-99.85%）。
结论和临床意义
SediVue 为人工方法检测尿液中的管型提供了中等程度的一致性。
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