比较 Anvajo Vet Fluidlab 1 尿液沉渣分析仪与手动显微镜和 IDEXX SediVue 分析用于分析猫和狗的尿液样本。
Comparison of the Anvajo Vet Fluidlab 1 urine sediment analyzer to manual microscopy and Idexx SediVue analysis for analysis of urine samples from cats and dogs.
机构信息
Clinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Ludwig Maximilian University, Munich, Germany.
出版信息
J Vet Diagn Invest. 2022 Nov;34(6):944-954. doi: 10.1177/10406387221124157. Epub 2022 Sep 14.
The Vet Fluidlab 1 (Anvajo), a new urine sediment analyzer for use in veterinary medicine, uses holographic microscopy to detect urine sediment particles in uncentrifuged urine. We compared the performance of the Fluidlab to manual microscopy and Idexx SediVue analysis for the detection of RBC, WBC, epithelial cells (EC), struvite crystals (STR), all crystals (CRY), and casts (CST) in urine samples from cats and dogs. The performance of the Fluidlab for the detection of bacteria was compared to bacterial culture. We included 624 urine samples from feline (238; 38%) and canine (386; 62%) patients; 227 samples had been submitted for bacterial culturing. The sensitivity of the Fluidlab compared to manual microscopy was 92.1% for RBC, 90.1% for WBC, 87.5% for EC, 67.6% for STR, 53.9% for CRY, and 12.5% for CST. Specificity was >97% for STR and CST, 90.0% for CRY, 78.4% for WBC, 59.4% for EC, and 55.1% for RBC. Sensitivities and specificities of the Fluidlab for analytes compared to manual microscopy were found to be similar to those obtained by the Fluidlab compared to SediVue analysis. Miscellaneous materials (e.g., lipid droplets, sperm, cell detritus) seemed to be the main reason for the high false-positive rate in RBC and EC classification by the Fluidlab. Detection of bacteria by the Fluidlab compared to bacterial culture had a sensitivity of 89.8% and a specificity of 72.3%. The performance of the Fluidlab is acceptable for the detection of WBC and bacteria; sensitivity for the detection of CRY and CST, and specificity for the detection of RBC and EC, require improvement.
兽医尿液分析系统 Fluidlab 1（Anvajo）是一款用于兽医诊断的新型尿液沉渣分析仪，它采用全息显微镜技术对未离心尿液中的沉渣颗粒进行检测。我们比较了 Fluidlab 与人工显微镜及 IDEXX SediVue 分析系统在猫和犬尿液样本中对红细胞（RBC）、白细胞（WBC）、上皮细胞（EC）、鸟粪石晶体（STR）、所有晶体（CRY）和管型（CST）的检测性能。我们还将 Fluidlab 对细菌的检测性能与细菌培养进行了比较。本研究纳入了 624 份来自猫（238 份，38%）和犬（386 份，62%）患者的尿液样本；其中 227 份样本进行了细菌培养。与人工显微镜相比，Fluidlab 对 RBC 的检测灵敏度为 92.1%，对 WBC 的检测灵敏度为 90.1%，对 EC 的检测灵敏度为 87.5%，对 STR 的检测灵敏度为 67.6%，对 CRY 的检测灵敏度为 53.9%，对 CST 的检测灵敏度为 12.5%。STR 和 CST 的特异性>97%，CRY 的特异性为 90.0%，WBC 的特异性为 78.4%，EC 的特异性为 59.4%，RBC 的特异性为 55.1%。Fluidlab 对分析物的检测灵敏度和特异性与 Fluidlab 与 SediVue 分析系统的比较结果相似。杂类物质（如脂质颗粒、精子、细胞碎片）似乎是导致 Fluidlab 对 RBC 和 EC 分类出现高假阳性率的主要原因。Fluidlab 对细菌的检测灵敏度为 89.8%，特异性为 72.3%。Fluidlab 对 WBC 和细菌的检测性能可以接受；CRY 和 CST 的检测灵敏度以及 RBC 和 EC 的检测特异性需要进一步提高。
Zotero 插件