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SERS 技术作为一种非核酸或抗原检测 SARS-CoV-2 病毒及其变体方法的挑战。

Challenges of SERS technology as a non-nucleic acid or -antigen detection method for SARS-CoV-2 virus and its variants.

机构信息

Engineered Materials for Biomedical Applications Laboratory, Department of Materials Science and Engineering, National Cheng Kung University, Tainan, 701, Taiwan.

Engineered Materials for Biomedical Applications Laboratory, Department of Materials Science and Engineering, National Cheng Kung University, Tainan, 701, Taiwan; Medical Device Innovation Center, National Cheng Kung University, 1 University Road, Tainan, 701, Taiwan.

出版信息

Biosens Bioelectron. 2021 Jun 1;181:113153. doi: 10.1016/j.bios.2021.113153. Epub 2021 Mar 9.

Abstract

The COVID-19 pandemic has caused a significant burden since December 2019 that has negatively impacted the global economy owing to the fact that the SARS-CoV-2 virus is fast-transmitting and highly contagious. Efforts have been taken to minimize the impact through strict screening measures in country borders in order to isolate potential virus carriers. Effective fast-screening methods are thus needed to identify infected individuals. The standard diagnostic methods for screening SARS-CoV-2 virus have always been to perform nucleic acid-based and serological tests. However, with each having drawbacks on producing false results at very early or later stage after symptoms onset, supplementary techniques are needed to back up these tests. Surface-enhanced Raman spectroscopy (SERS) as a detection technique has continuously advanced throughout the years in terms of sensitivity and capability to detect ultralow concentration of analytes ranging from single molecule to pathogens, to present as a highly potential alternative to known sensing methods. SERS technology as a candidate for an alternative and supplementary diagnostic method for the viral envelope of SARS-CoV-2 virus is presented, comparing its pros and cons to the standard methods and what other aspects it could offer that the other methods are not capable of. Factors that contribute to the detection effectivity of SERS is also discussed to show the advantages and limitations of this technique. Despite its promising capabilities, challenges like sources of SARS-CoV-2 virus and its variations, reliable SERS spectra, mass production of SERS-active substrates, and compliance to regulations for wide-scale testing scenario are highlighted.

摘要

自 2019 年 12 月以来,COVID-19 大流行造成了巨大的负担,由于 SARS-CoV-2 病毒传播速度快且具有高度传染性,对全球经济造成了负面影响。为了尽量减少这种影响,人们在国家边境采取了严格的筛选措施,以隔离潜在的病毒携带者。因此,需要有效的快速筛选方法来识别感染者。筛查 SARS-CoV-2 病毒的标准诊断方法一直是进行基于核酸和血清学的检测。然而,由于这两种方法在症状出现的早期或晚期都有可能产生假阳性结果,因此需要辅助技术来支持这些检测。表面增强拉曼光谱(SERS)作为一种检测技术,在灵敏度和检测从单分子到病原体等超低浓度分析物的能力方面,多年来一直在不断进步,是一种很有前途的替代已知传感方法的选择。本文提出了 SERS 技术作为 SARS-CoV-2 病毒包膜的替代和辅助诊断方法的候选,比较了其与标准方法的优缺点,以及它在其他方法无法提供的方面所能提供的优势。还讨论了影响 SERS 检测效果的因素,以展示该技术的优势和局限性。尽管 SERS 具有很大的应用潜力,但仍存在一些挑战,如 SARS-CoV-2 病毒的来源及其变异、可靠的 SERS 光谱、SERS 活性衬底的大规模生产以及符合大规模检测场景的法规等问题。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd08/7939978/ce9083174910/gr1_lrg.jpg

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