Karpathiou Georgia, Dumollard Jean Marc, Camy Florian, Sramek Viviana, Dridi Maroa, Picot Tiphanie, Mobarki Mousa, Peoc'h Michel
Pathology Department, University Hospital of Saint-Etienne, Saint-Priest-en-Jarez, France.
Pathology Department, University Hospital of Saint-Etienne, Saint-Priest-en-Jarez, France.
Cardiovasc Pathol. 2021 May-Jun;52:107335. doi: 10.1016/j.carpath.2021.107335. Epub 2021 Mar 21.
Cardiac myxomas are rare tumors of incompletely elucidated pathogenesis. The aim of this study is to explore the possible presence of a senescence phenotype in cardiac myxomas, associated with an inflammatory and vasculogenic tumor microenvironment.
This is a retrospective study of 29 cardiac myxomas with immunohistochemical detection of various inflammatory, vascular, and senescence markers. We show that all myxomas contain tumor cells in senescence overexpressing p16, and a fraction of senescent endothelial cells. Macrophages are the principal inflammatory cell population, followed by cytotoxic T cells, with fewer plasma cells, mastocytes, and B lymphocytes. These populations are found in different intratumoral localizations. Larger tumor volume is associated with a lower percentage of myxoid matrix, higher cellularity, higher macrophage, and lower number of mast cells as well as higher PD-L1 expression by inflammatory cells. Higher vascular density is associated with higher percentage of B cells, a lower number of macrophages and higher number of mastocytes, and lower PD-L1 expression by inflammatory cells. Tumors with higher vascular density and higher cellularity show higher amounts of p16 senescent endothelial cells.
Myxoma tumor cells are in senescence and reside inside a tumor microenvironment with a distinct inflammatory profile rich in macrophages and cytotoxic T cells, and a rich vasculature, probably attributed to a senescence-associated secretory phenotype.
心脏黏液瘤是一种发病机制尚未完全阐明的罕见肿瘤。本研究的目的是探讨心脏黏液瘤中可能存在的衰老表型，以及与炎症和血管生成性肿瘤微环境的相关性。
这是一项对29例心脏黏液瘤的回顾性研究，通过免疫组织化学检测各种炎症、血管和衰老标志物。我们发现所有黏液瘤均含有过表达p16的衰老肿瘤细胞以及一部分衰老内皮细胞。巨噬细胞是主要的炎症细胞群体，其次是细胞毒性T细胞，浆细胞、肥大细胞和B淋巴细胞较少。这些细胞群体分布于肿瘤内不同部位。肿瘤体积较大与黏液样基质比例较低、细胞密度较高、巨噬细胞比例较高、肥大细胞数量较少以及炎症细胞中PD-L1表达较高相关。血管密度较高与B细胞比例较高、巨噬细胞数量较少、肥大细胞数量较多以及炎症细胞中PD-L1表达较低相关。血管密度较高且细胞密度较高的肿瘤显示p16衰老内皮细胞数量较多。
黏液瘤肿瘤细胞处于衰老状态，存在于具有独特炎症特征（富含巨噬细胞和细胞毒性T细胞）和丰富血管系统的肿瘤微环境中，这可能归因于衰老相关分泌表型。
