Sharma Mruganxi Harshad, Palamae Suriya, Yingkajorn Mingkwan, Benjakul Soottawat, Singh Avtar, Buatong Jirayu
International Center of Excellence in Seafood Science and Innovation, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Foods. 2024 Jul 27;13(15):2375. doi: 10.3390/foods13152375.
spp. is a Gram-negative bacteria known for its ability to cause foodborne infection in association with eating raw or undercooked seafood. The majority of these foodborne illnesses are caused by mollusks, especially bivalves. Thus, the prevalence of spp. in blood clams (), baby clams (), and Asian green mussels () from South Thailand was determined. A total of 649 spp. isolates were subjected to pathogenicity analysis on blood agar plates, among which 21 isolates from blood clams (15 isolates), baby clams (2 isolates), and green mussels (4 isolates) showed positive -hemolysis. Based on the biofilm formation index (BFI) of -hemolysis-positive strains, nine isolates exhibited a strong biofilm formation capacity, with a BFI in the range of 1.37 to 10.13. Among the 21 isolates, 6 isolates (BL18, BL82, BL84, BL85, BL90, and BL92) were -positive, while and genes were not detected in all strains. Out of 21 strains, 5 strains showed multidrug resistance (MDR) against amoxicillin/clavulanic acid, ampicillin/sulbactam, cefotaxime, cefuroxime, meropenem, and trimethoprim/sulfamethoxazole. A phylogenetic analysis of MDR was performed based on 16s rDNA sequences using the neighbor-joining method. The five MDR isolates were identified to be (one isolate) (one isolate) and, (three isolates). In addition, the antimicrobial activity of chitooligosaccharide-epigallocatechin gallate (COS-EGCG) conjugate against MDR strains was determined. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of COS-EGCG conjugate were in the range of 64-128 µg/mL. The antimicrobial activity of the conjugate was advocated by the cell lysis of MDR strains, as elucidated by scanning electron microscopic images. spp. isolated from blood clams, baby clams, and Asian green mussels were highly pathogenic, exhibiting the ability to produce biofilm and being resistant to antibiotics. However, the COS-EGCG conjugate could be used as a potential antimicrobial agent for controlling in mollusks.
某菌属是一种革兰氏阴性菌，以其与食用生的或未煮熟的海鲜相关联而导致食源性感染的能力而闻名。这些食源性疾病大多由软体动物引起，尤其是双壳贝类。因此，测定了泰国南部血蚶、小贝和亚洲绿贻贝中该菌属的流行情况。总共649株该菌属分离株在血琼脂平板上进行致病性分析，其中来自血蚶的21株分离株（15株）、小贝的2株分离株和绿贻贝的4株分离株显示出阳性β-溶血。基于β-溶血阳性菌株的生物膜形成指数（BFI），9株分离株表现出很强的生物膜形成能力，BFI在1.37至10.13范围内。在这21株分离株中，6株（BL18、BL82、BL84、BL85、BL90和BL92）为某基因阳性，而所有菌株中均未检测到其他基因。在21株菌株中，5株对阿莫西林/克拉维酸、氨苄西林/舒巴坦、头孢噻肟、头孢呋辛、美罗培南和甲氧苄啶/磺胺甲恶唑表现出多重耐药（MDR）。基于16s rDNA序列，采用邻接法对多重耐药某菌属进行了系统发育分析。这5株多重耐药分离株被鉴定为某菌（1株）、另一菌（1株）和又一菌（3株）。此外，还测定了壳寡糖-表没食子儿茶素没食子酸酯（COS-EGCG）缀合物对多重耐药某菌属菌株的抗菌活性。COS-EGCG缀合物的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）在64 - 128 µg/mL范围内。扫描电子显微镜图像表明，缀合物的抗菌活性是通过多重耐药某菌属菌株的细胞裂解来体现的。从血蚶、小贝和亚洲绿贻贝中分离出的某菌属具有高度致病性，表现出产生生物膜的能力且对抗生素耐药。然而，COS-EGCG缀合物可作为控制软体动物中某菌属的潜在抗菌剂。
