Fang Jianjun, Wang Dan, Hu FangFang, Li Xinru, Zou Xiaotong, Xie Jinlu, Zhou Zhihua
Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of the Ministry of Education, Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, Hunan 411201, PR China; College of Animal Science, Zhejiang University, Hangzhou, Zhejiang 310058, PR China.
Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of the Ministry of Education, Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, Hunan 411201, PR China.
Mater Sci Eng C Mater Biol Appl. 2021 Sep;128:112354. doi: 10.1016/j.msec.2021.112354. Epub 2021 Jul 31.
In this paper, silk fibroin (SF) porous microcarriers containing strontium were constructed as injectable bone tissue engineering vehicles. The effects of SF concentration and strontium content on micromorphology, element distribution, strontium ion release and cellular behavior of the constructed microcarriers were investigated. The microcarriers with an open interconnected pore can be fabricated by controlling the concentration of SF. The strontium functionalized SF microcarriers showed the sustained release of strontium ion and allowed bone mesenchymal stem cells (BMSCs) to attach, proliferate and secrete extracellular matrix. Furthermore, the strontium functionalized SF microcarriers improved the osteogenic capability of BMSCs in vitro compared with those microcarriers without sustained release of strontium ion. This study presents a valuable approach to fabricate polymeric microcarriers with the capability of sustained release of strontium ion that show potential in bone tissue engineering applications.
在本文中，构建了含锶的丝素蛋白（SF）多孔微载体作为可注射骨组织工程载体。研究了SF浓度和锶含量对构建的微载体微观形态、元素分布、锶离子释放及细胞行为的影响。通过控制SF浓度可制备具有开放互连孔的微载体。锶功能化的SF微载体表现出锶离子的持续释放，并能使骨髓间充质干细胞（BMSC）附着、增殖并分泌细胞外基质。此外，与无锶离子持续释放的微载体相比，锶功能化的SF微载体在体外提高了BMSC的成骨能力。本研究提出了一种制备具有锶离子持续释放能力的聚合物微载体的有价值方法，该微载体在骨组织工程应用中显示出潜力。
