Zhang Yumin, Alvarez-Manzo Hector, Leone Jacob, Schweig Sunjya, Zhang Ying
Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States.
FOCUS Health Group, Naturopathic, Novato, CA, United States.
Front Cell Infect Microbiol. 2021 Mar 8;11:624745. doi: 10.3389/fcimb.2021.624745. eCollection 2021.
Human babesiosis is a CDC reportable disease in the United States and is recognized as an emerging health risk in multiple parts of the world. The current treatment for human babesiosis is suboptimal due to treatment failures and unwanted side effects. Although was first described almost 30 years ago, further research is needed to elucidate its pathogenesis and clarify optimal treatment regimens. Here, we screened a panel of herbal medicines and identified , , , and to have good inhibitory activity against in the hamster erythrocyte model. Furthermore, we found their potential bioactive compounds, cryptolepine, artemisinin, artesunate, artemether, and baicalein, to have good activity against , with IC values of 3.4 μM, 14 μM, 7.4 μM, 7.8 μM, and 12 μM, respectively, which are comparable or lower than that of the currently used drugs quinine (10 μM) and clindamycin (37 μM). treated with cryptolepine and quinine at their respective 1×, 2×, 4× and 8× IC values, and by artemether at 8× IC for three days could not regrow in subculture. Additionally, 90% ethanol extract also exhibited no regrowth after 6 days of subculture at doses of 2×, 4×, and 8× IC values. Our results indicate that some botanical medicines and their active constituents have potent activity against and may be further explored for more effective treatment of babesiosis.
人巴贝斯虫病在美国是一种可向疾病控制与预防中心报告的疾病，并且在世界多个地区被认为是一种新出现的健康风险。由于治疗失败和不良副作用，目前针对人巴贝斯虫病的治疗并不理想。尽管该病于近30年前首次被描述，但仍需要进一步研究以阐明其发病机制并明确最佳治疗方案。在此，我们筛选了一组草药，发现在仓鼠红细胞模型中，[草药名称1]、[草药名称2]、[草药名称3]和[草药名称4]对[巴贝斯虫名称]具有良好的抑制活性。此外，我们发现它们的潜在生物活性化合物隐丹参酮、青蒿素、青蒿琥酯、蒿甲醚和黄芩苷对[巴贝斯虫名称]具有良好活性，其IC值分别为3.4 μM、14 μM、7.4 μM、7.8 μM和12 μM，与目前使用的药物奎宁（10 μM）和克林霉素（37 μM）相当或更低。用隐丹参酮和奎宁分别以其1×、2×、4×和8× IC值处理，以及用蒿甲醚以8× IC值处理三天后的[巴贝斯虫名称]在传代培养中无法再生长。此外，[草药名称]90%乙醇提取物在以2×、4×和8× IC值剂量传代培养6天后也未出现再生长。我们的结果表明，一些植物药及其活性成分对[巴贝斯虫名称]具有强大活性，可能值得进一步探索用于更有效地治疗巴贝斯虫病。
