Clinical Laboratory of Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
Biomed Res Int. 2021 Mar 8;2021:5450397. doi: 10.1155/2021/5450397. eCollection 2021.
We aimed to establish a method to determine whether microRNA-193b (miR-193b) levels in ABCA1-labeled serum exosomes might serve as a marker for the diagnosis of Alzheimer's disease.
We used immunocapture methods to determine the levels of ABCA1-labeled exosomal miR-193b in cultures of white blood cells (WBCs), red blood cells (RBCs), mouse hippocampal neuron HT-22 cells, and primary mouse neuronal cells. ABCA1-labeled exosomal miR-193b levels were also evaluated in the cerebrospinal fluid (CSF) and serum of APP/PS1 double-transgenic mice, as well as control subjects ( = 60) and study participants with subjective cognitive decline (SCD, = 89), stage and mild cognitive impairment (MCI, = 92), and dementia of the Alzheimer type (DAT, = 92).
ABCA1 levels of exosomes harvested from the medium of HT-22 cells and neurons were significantly higher than those of RBCs and WBCs ( < 0.05). Exosomal ABCA1 from the CSF of APP/PS1 mice were transmitted to the serum of wild-type mice after injection, and high miR-193b levels were observed in both the serum and CSF after injection. The ABCA1-labeled exosomal miR-193b levels were higher in the CSF of MCI and DAT patients compared with the CSF of the control group ( < 0.05). The ABCA1-labeled exosomal miR-193b were also slightly higher ( > 0.05) in the serum of SCD patients and significantly higher in the serum of MCI and DAT patients compared with the serum of the control group ( < 0.05).
This study provides a method to capture specific exosomes. Detection of serum exosomes labeled with ABCA1 may facilitate the early diagnosis of AD.
本研究旨在建立一种方法，以确定载 ABCA1 的血清外泌体中的 microRNA-193b（miR-193b）水平是否可作为诊断阿尔茨海默病的标志物。
我们使用免疫捕获方法测定白细胞（WBC）、红细胞（RBC）、鼠海马神经元 HT-22 细胞和原代鼠神经元细胞培养物中载 ABCA1 的外泌体 miR-193b 的水平。还评估了 APP/PS1 双转基因小鼠以及对照受试者（n=60）和有主观认知下降（SCD，n=89）、轻度认知障碍（MCI，n=92）和阿尔茨海默病型痴呆（DAT，n=92）的研究参与者的脑脊液（CSF）和血清中的载 ABCA1 的外泌体 miR-193b 水平。
HT-22 细胞和神经元来源的外泌体中 ABCA1 水平明显高于 RBC 和 WBC（P<0.05）。APP/PS1 小鼠 CSF 中的外泌体 ABCA1 在注射后被传递到野生型小鼠的血清中，并且注射后在血清和 CSF 中均观察到高 miR-193b 水平。与对照组相比，MCI 和 DAT 患者的 CSF 中的载 ABCA1 的外泌体 miR-193b 水平更高（P<0.05）。SCD 患者血清中的载 ABCA1 的外泌体 miR-193b 也略高（P>0.05），而 MCI 和 DAT 患者的血清中的载 ABCA1 的外泌体 miR-193b 水平则明显高于对照组（P<0.05）。
本研究提供了一种捕获特定外泌体的方法。检测载 ABCA1 的血清外泌体可能有助于 AD 的早期诊断。
