State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, People's Republic of China.
State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, People's Republic of China.
Arch Microbiol. 2021 Aug;203(6):2911-2917. doi: 10.1007/s00203-021-02280-z. Epub 2021 Mar 24.
A novel Gram-staining negative, crescent-like or rod-shaped, non-motile bacterium, designated strain CUG 91378, was isolated from a sediment sample of Qinghai Lake, Qinghai Province, China. The strain was red-colored, and catalase- and oxidase-positive. Strain CUG 91378 was able to grow at 15-37 °C (optimum, 28 °C), pH 7-9 (pH 7.0) and in the presence of up to 3.0% (w/v) NaCl (0-2%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CUG 91378 formed a well-supported monophyletic clade with Aquiflexum balticum DSM 16537 (95.4%) and Aquiflexum aquatile Z0201 (93.2%). The DNA G + C content of CUG 91378 was 39.0%. Low (< 87%) average nucleotide identity (ANI) and (< 26%) digital DNA-DNA hybridization (dDDH) values were observed between strain CUG 91378 and its closest species on the phylogenetic trees. The sole respiratory quinone of strain CUG 91378 was MK-7. The predominant fatty acids (> 5.0%) were iso-C (19.1%), iso-C (12.0%), iso-C H (10.9%), iso-C 3OH (9.2%), iso-C 3OH (7.7%), Cω6c (6.1%) and anteiso-C (5.8%). Strain CUG 91378 contained as phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and four unidentified lipids (L1, L2, L3 and L4). Based on the data from the current polyphasic study, the isolate represents a novel species of the genus Aquiflexum for which the name Aquiflexum lacus is proposed. The type strain of the proposed new taxon is CUG 91378 (= KCTC 62637 = CGMCC 1.13988).
从中国青海省青海湖的沉积物样本中分离到一株革兰氏染色阴性、新月形或杆状、非运动的新型细菌，命名为 CUG 91378 菌株。该菌株呈红色，过氧化氢酶和氧化酶阳性。CUG 91378 菌株能够在 15-37°C（最佳温度 28°C）、pH7-9（pH7.0）和高达 3.0%（w/v）NaCl（0-2%）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，CUG 91378 菌株与 Aquiflexum balticum DSM 16537（95.4%）和 Aquiflexum aquatile Z0201（93.2%）形成一个支持度高的单系分支。CUG 91378 的 DNA G+C 含量为 39.0%。在系统发育树上，CUG 91378 菌株与其最接近的种之间的平均核苷酸同一性（ANI）值低于 87%，数字 DNA-DNA 杂交（dDDH）值低于 26%。CUG 91378 菌株的唯一呼吸醌为 MK-7。主要脂肪酸（>5.0%）为 iso-C（19.1%）、iso-C（12.0%）、iso-C H（10.9%）、iso-C 3OH（9.2%）、iso-C 3OH（7.7%）、Cω6c（6.1%）和 anteiso-C（5.8%）。CUG 91378 菌株含有磷脂酰乙醇胺（PE）、磷脂酰甘油（PG）和四种未鉴定的脂质（L1、L2、L3 和 L4）。根据当前多相研究的数据，该分离株代表 Aquiflexum 属的一个新型种，建议将其命名为 Aquiflexum lacus。提议的新分类单元的模式株为 CUG 91378（=KCTC 62637=CGMCC 1.13988）。
