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酶联免疫吸附测定法与补体结合试验在口蹄疫和猪水疱病诊断中的常规应用比较

Routine application of enzyme-linked immunosorbent assay in comparison with complement fixation for the diagnosis of foot-and-mouth and swine vesicular diseases.

作者信息

Ferris N P, Dawson M

机构信息

Institute for Animal Disease Research, Pirbright Laboratory, Surrey, Gt. Britain.

出版信息

Vet Microbiol. 1988 Mar;16(3):201-9. doi: 10.1016/0378-1135(88)90024-7.

Abstract

An indirect, sandwich enzyme-linked immunosorbent assay (ELISA) has been compared in parallel with the standard complement-fixation typing test (CFT) for the diagnosis of foot-and-mouth and swine vesicular diseases. The superior sensitivity, reproducibility, economical use of reagents and ease of performance of the ELISA confirm the hypothesis that it should replace the CFT as the routine test of choice.

摘要

已将一种间接夹心酶联免疫吸附测定法(ELISA)与标准补体结合分型试验(CFT)进行了平行比较,用于口蹄疫和猪水泡病的诊断。ELISA具有更高的灵敏度、可重复性、试剂使用经济且操作简便,证实了它应取代CFT作为常规首选检测方法的假设。

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