一种用于口蹄疫初步诊断的酶联免疫吸附测定（ELISA）。特性及与补体结合试验的比较。
An enzyme-linked immunosorbent assay (ELISA) for the primary diagnosis of foot-and-mouth disease. Characterization and comparison with complement fixation.
作者信息
Have P, Lei J C, Schjerning-Thiesen K
出版信息
Acta Vet Scand. 1984;25(2):280-96. doi: 10.1186/BF03547271.
Abstract
A sandwich type ELISA for foot-and-mouth disease (FMD) virus types O, A and C was established, using a combination of rabbit anti-146 S and guinea pig hyperimmune antibodies. This method was found to be highly efficient for the detection of both 146 S particles and 12 S subunits. The ELISA was approximately 500 times more sensitive than complement fixation (CF) when examining epithelial samples of FMD vesicles. An early primary diagnosis of FMD was obtained by both CF and ELISA in 19 out of 21 confirmed cases. The remaining 2 cases were initially negative in CF but positive in ELISA.
摘要
利用兔抗146 S抗体和豚鼠超免疫抗体组合，建立了一种用于检测口蹄疫病毒O型、A型和C型的夹心型ELISA方法。结果发现该方法在检测146 S颗粒和12 S亚基方面效率很高。在检测口蹄疫水疱上皮样本时，该ELISA方法的灵敏度比补体结合试验（CF）高约500倍。在21例确诊病例中，CF和ELISA在19例中均获得了口蹄疫的早期初步诊断。其余2例最初CF检测为阴性，但ELISA检测为阳性。
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本文引用的文献
1
A study of the cross-reacting antigens on the intact foot-and-mouth disease virus and its 12S Subunits with antisera against the structural proteins.一项关于完整口蹄疫病毒及其12S亚基上与抗结构蛋白抗血清发生交叉反应的抗原的研究。
J Gen Virol. 1980 Nov;51(Pt 1):107-16. doi: 10.1099/0022-1317-51-1-107.
2
Serological and immunological relations between the 146S and 12S particles of foot-and-mouth disease virus.口蹄疫病毒146S和12S颗粒之间的血清学和免疫学关系。
J Gen Virol. 1980 Oct;50(2):369-75. doi: 10.1099/0022-1317-50-2-369.
3
Monoclonal antibodies against foot-and-mouth disease virus 146S and 12S particles.针对口蹄疫病毒146S和12S颗粒的单克隆抗体。
Arch Virol. 1982;74(1):1-9. doi: 10.1007/BF01320777.
4
Neutralization of foot-and-mouth disease virus. I. Sensitization of the 140S virion by antibody also reactive with the 12S protein subunit.口蹄疫病毒的中和作用。I. 抗体对140S病毒粒子的致敏作用，该抗体也与12S蛋白质亚基发生反应。
J Gen Virol. 1981 Aug;55(Pt 2):415-27. doi: 10.1099/0022-1317-55-2-415.
5
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