一种通过（鼠×牛）×牛种间融合制备的针对雌酮/雌二醇的牛单克隆抗体。
A bovine monoclonal antibody to oestrone/oestradiol prepared by a (murine x bovine) xbovine interspecies fusion.
作者信息
Groves D J, Clayton J, Morris B A
机构信息
A.F.R.C. Antibody Development Group, Department of Biochemistry, University of Surrey, Guildford, Great Britain.
出版信息
Vet Immunol Immunopathol. 1988 Feb;18(1):95-101. doi: 10.1016/0165-2427(88)90039-6.
Abstract
A heterohybridoma was produced by the fusion of sensitized peripheral blood lymphocytes (PBLs) with a previously derived heteromyeloma, generated by the fusion of bovine PBLs with murine myeloma cells. The sensitized bovine PBLs were collected from a steer immunized with an oestradiol-ovalbumin conjugate. The cell lines resulting from the fusion were screened for the production of bovine antibodies to oestradiol. A stable heterohybridoma was isolated which secreted a bovine IgG1 to oestrone/oestradiol. The use of sensitized PBLs together with heteromyeloma fusion partners has proved to be a reliable and simple way of producing monoclonal antibodies against specific haptens.
摘要
通过将致敏外周血淋巴细胞（PBL）与先前获得的异源骨髓瘤融合产生了异源杂交瘤，该异源骨髓瘤是由牛PBL与鼠骨髓瘤细胞融合产生的。致敏牛PBL取自用雌二醇-卵清蛋白偶联物免疫的公牛。对融合产生的细胞系进行筛选，以检测其是否产生抗雌二醇的牛抗体。分离出一种稳定的异源杂交瘤，它分泌针对雌酮/雌二醇的牛IgG1。事实证明，将致敏PBL与异源骨髓瘤融合伙伴一起使用是生产针对特定半抗原的单克隆抗体的一种可靠且简单的方法。
相似文献
1
A bovine monoclonal antibody to oestrone/oestradiol prepared by a (murine x bovine) xbovine interspecies fusion.一种通过（鼠×牛）×牛种间融合制备的针对雌酮/雌二醇的牛单克隆抗体。
Vet Immunol Immunopathol. 1988 Feb;18(1):95-101. doi: 10.1016/0165-2427(88)90039-6.
2
3
Generation by self re-fusion of bovine³ × murine² heterohybridomas secreting virus-neutralizing bovine monoclonal antibodies to bovine herpesvirus 1 glycoproteins gB, gC, and gD.通过分泌针对牛疱疹病毒1糖蛋白gB、gC和gD的病毒中和牛单克隆抗体的牛³×鼠²异源杂交瘤自身重融合产生。
Vet Immunol Immunopathol. 2014 May 15;159(1-2):58-73. doi: 10.1016/j.vetimm.2014.02.004. Epub 2014 Feb 16.
4
Specific bovine monoclonal antibody produced by a re-fused mouse/calf hybridoma.由重新融合的小鼠/小牛杂交瘤产生的特异性牛单克隆抗体。
Hybridoma. 1984 Summer;3(2):171-6. doi: 10.1089/hyb.1984.3.171.
5
Murine/bovine hybridomas producing monoclonal alloantibodies to bovine red cell antigens.
Anim Genet. 1987;18(1):29-39. doi: 10.1111/j.1365-2052.1987.tb00741.x.
6
Construction of a bovine-murine heteromyeloma cell line; production of bovine monoclonal antibodies against rotavirus and pregnant mare serum gonadotrophin.牛-鼠杂交骨髓瘤细胞系的构建；抗轮状病毒和孕马血清促性腺激素的牛单克隆抗体的制备
Vet Immunol Immunopathol. 1990 Mar;24(3):211-26. doi: 10.1016/0165-2427(90)90037-s.
7
Characterization of heteromyeloma fusion partners which promote the outgrowth of porcine hybridomas.促进猪杂交瘤生长的异骨髓瘤融合伙伴的特性分析。
Vet Immunol Immunopathol. 1990 Nov;26(3):267-83. doi: 10.1016/0165-2427(90)90096-b.
8
Production and characterization of anti-estrone monoclonal antibody.抗雌酮单克隆抗体的制备与鉴定
Biomed Environ Sci. 2002 Jun;15(2):103-12.
9
Production of stable bovine-murine interspecies hybrids.
Methods Mol Biol. 1995;45:41-7. doi: 10.1385/0-89603-308-2:41.
10
Production of monoclonal antibodies to bovine leukocyte cell-surface components.抗牛白细胞细胞表面成分单克隆抗体的制备。
J Dairy Sci. 1983 Jul;66(7):1547-58. doi: 10.3168/jds.S0022-0302(83)81971-7.
引用本文的文献
1
Rational monoclonal antibody development to emerging pathogens, biothreat agents and agents of foreign animal disease: The antigen scale.针对新出现的病原体、生物威胁因子和外来动物疾病病原体的合理单克隆抗体开发：抗原规模。
Vet J. 2005 Sep;170(2):193-211. doi: 10.1016/j.tvjl.2004.04.021.
2
The production and application of non-rodent monoclonal antibodies in veterinary science.非啮齿类单克隆抗体在兽医学中的生产与应用。
Vet Immunol Immunopathol. 1989 Nov 30;23(1-2):1-14. doi: 10.1016/0165-2427(89)90105-0.
3
Construction of a bovine-murine heteromyeloma cell line; production of bovine monoclonal antibodies against rotavirus and pregnant mare serum gonadotrophin.牛-鼠杂交骨髓瘤细胞系的构建；抗轮状病毒和孕马血清促性腺激素的牛单克隆抗体的制备
Vet Immunol Immunopathol. 1990 Mar;24(3):211-26. doi: 10.1016/0165-2427(90)90037-s.
4
Bovine viral diarrhoea virus-specific bovine monoclonal antibody.牛病毒性腹泻病毒特异性牛单克隆抗体
Arch Virol. 1991;121(1-4):219-25. doi: 10.1007/BF01316756.
本文引用的文献
1
Calculation of average antibody affinity in anti-hapten sera from data obtained by competitive radioimmunoassay.根据竞争性放射免疫分析所获数据计算抗半抗原血清中的平均抗体亲和力。
J Immunol Methods. 1980;34(4):345-52. doi: 10.1016/0022-1759(80)90107-6.
2
Improvement of sheep fecundity by treatment with antisera to gonadal steroids.
J Reprod Fertil. 1982 Nov;66(2):625-34. doi: 10.1530/jrf.0.0660625.
3
4
Specific bovine monoclonal antibody produced by a re-fused mouse/calf hybridoma.由重新融合的小鼠/小牛杂交瘤产生的特异性牛单克隆抗体。
Hybridoma. 1984 Summer;3(2):171-6. doi: 10.1089/hyb.1984.3.171.
5
Concentration of gonadotrophins in the plasma of sheep given gonadal steroid antisera to raise ovulation rate.
J Reprod Fertil. 1984 Sep;72(1):93-100. doi: 10.1530/jrf.0.0720093.
6
Production and characterization of monoclonal bovine immunoglobulins G1, G2 and M from bovine x murine hybridomas.源自牛-鼠杂交瘤的单克隆牛免疫球蛋白G1、G2和M的制备与特性分析
Vet Immunol Immunopathol. 1984 Mar;5(4):323-42. doi: 10.1016/0165-2427(84)90002-3.
7
Construction and testing of mouse--human heteromyelomas for human monoclonal antibody production.用于生产人单克隆抗体的小鼠-人异种骨髓瘤的构建与测试。
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7308-12. doi: 10.1073/pnas.80.23.7308.
8
Monoclonal antibodies for diagnosis of infectious diseases in humans.用于人类传染病诊断的单克隆抗体。
Science. 1983 Feb 11;219(4585):637-44. doi: 10.1126/science.6297006.
9
Somatic cell hybridization between bovine leukemia virus-infected lymphocytes and murine plasma cell tumors: cell fusion studies with bovine cells.
Mol Immunol. 1982 Jul;19(7):893-9. doi: 10.1016/0161-5890(82)90355-8.
10
A graphic method for the determination and presentation of binding parameters in a complex system.一种用于确定和呈现复杂系统中结合参数的图形方法。
Anal Biochem. 1967 Sep;20(3):525-32. doi: 10.1016/0003-2697(67)90297-7.
Zotero 插件