Groves D J, Clayton J, Morris B A
A.F.R.C. Antibody Development Group, Department of Biochemistry, University of Surrey, Guildford, Great Britain.
Vet Immunol Immunopathol. 1988 Feb;18(1):95-101. doi: 10.1016/0165-2427(88)90039-6.
A heterohybridoma was produced by the fusion of sensitized peripheral blood lymphocytes (PBLs) with a previously derived heteromyeloma, generated by the fusion of bovine PBLs with murine myeloma cells. The sensitized bovine PBLs were collected from a steer immunized with an oestradiol-ovalbumin conjugate. The cell lines resulting from the fusion were screened for the production of bovine antibodies to oestradiol. A stable heterohybridoma was isolated which secreted a bovine IgG1 to oestrone/oestradiol. The use of sensitized PBLs together with heteromyeloma fusion partners has proved to be a reliable and simple way of producing monoclonal antibodies against specific haptens.
通过将致敏外周血淋巴细胞（PBL）与先前获得的异源骨髓瘤融合产生了异源杂交瘤，该异源骨髓瘤是由牛PBL与鼠骨髓瘤细胞融合产生的。致敏牛PBL取自用雌二醇-卵清蛋白偶联物免疫的公牛。对融合产生的细胞系进行筛选，以检测其是否产生抗雌二醇的牛抗体。分离出一种稳定的异源杂交瘤，它分泌针对雌酮/雌二醇的牛IgG1。事实证明，将致敏PBL与异源骨髓瘤融合伙伴一起使用是生产针对特定半抗原的单克隆抗体的一种可靠且简单的方法。
