Kiefel V, Santoso S, Katzmann B, Mueller-Eckhardt C
Institute for Clinical Immunology, Justus Liebig University, Giessen, FRG.
Vox Sang. 1988;54(2):101-6. doi: 10.1111/j.1423-0410.1988.tb01625.x.
Sera obtained from 4 mothers of children with neonatal alloimmune thrombocytopenia contained a platelet-specific alloantibody, anti-Bra, which defined an antigen apparently different from all known platelet alloantigens. All 4 fathers were Bra positive, whereas all mothers were Bra negative. The minimal postnatal values of platelet counts ranged from 19 X 10(9) to 75 X 10(9)/1. Family studies showed that the Bra antigen is inherited as an autosomal, codominant trait. Its antigen frequency in the German population is 20% (21 of 105 unrelated donors were positive). The estimated gene frequency is 0.11. The antibodies were identified by a glycoprotein-specific enzyme immunoassay using monoclonal antibodies for antigen immobilization, while they could not reliably be detected by binding assays employing whole platelets (platelet immunofluorescence, indirect competitive ELISA).
从4名新生儿同种免疫性血小板减少症患儿的母亲体内获取的血清中含有一种血小板特异性同种抗体——抗-Bra，该抗体所定义的抗原明显不同于所有已知的血小板同种抗原。4名患儿的父亲均为Bra阳性，而所有母亲均为Bra阴性。血小板计数的最低产后值范围为19×10⁹至75×10⁹/升。家系研究表明，Bra抗原作为常染色体共显性性状遗传。其在德国人群中的抗原频率为20%（105名无关供者中有21名呈阳性）。估计基因频率为0.11。这些抗体通过使用单克隆抗体固定抗原的糖蛋白特异性酶免疫测定法进行鉴定，而采用全血小板的结合测定法（血小板免疫荧光、间接竞争ELISA）无法可靠地检测到这些抗体。
