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是物种中快速的多糖生产者。 (这段英文表述不太完整准确,翻译出来的中文也稍显生硬,但完全按照要求进行了翻译)

is a fast polysaccharide producer among species.

作者信息

Viceconte Fátima R, Diaz Marina L, Soresi Daniela S, Lencinas Ileana B, Carrera Alicia, Prat María Inés, Gurovic María Soledad Vela

机构信息

Centro de Recursos Renovables de la Zona Semiárida (CERZOS), Universidad Nacional del Sur-CONICET, Camino La Carrindanga Km7, B8000 Bahía Blanca, Buenos Aires, Argentina.

Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, San Juan 670, B8000 Bahía Blanca, Buenos Aires, Argentina.

出版信息

Mycologia. 2021 May-Jun;113(3):513-524. doi: 10.1080/00275514.2020.1870255. Epub 2021 Mar 25.

DOI:10.1080/00275514.2020.1870255
PMID:33764859
Abstract

The selection of fast-growing and high-yield-producing strains is required to satisfy the market demand on fungal food supplements. To that aim, three strains deposited in our collection as and were screened for polysaccharide production and biomass yield. strains deposited as were identified as and by nuc rDNA internal transcribed spacer ITS1-5.8S-ITS2 (ITS) and translation elongation factor 1-α () phylogenies. The identity of was confirmed by molecular phylogeny and biogeography. Additionally, mycelial antagonism confirmed species differentiation, and strains were further distinguished by morphology and protein profiles. Biomass and polysaccharide yields of were clearly different from those of and in both liquid culture and solid-state fermentation. The maximum polysaccharide yield (4.52 ± 0.83 g L) for was obtained from submerged cultures at day 9. also achieved the highest linear growth in lignocellulosic solid substrates. Consequently, basidiomata were successfully obtained by solid-state fermentation in polypropylene bags, whereas and mushrooms were not produced in artificial solid substrates. , a species frequently collected in America, showed to be a promising polysaccharide producer for the manufacture of dietary supplements.

摘要

为满足市场对真菌类食品补充剂的需求,需要选择生长快、产量高的菌株。为此,对保藏于我们菌种库中的三株分别编号为[具体编号]的菌株进行了多糖产量和生物量产量的筛选。通过核糖体DNA内转录间隔区ITS1-5.8S-ITS2(ITS)和翻译延伸因子1-α([具体名称])系统发育分析,将编号为[具体编号]的菌株鉴定为[具体菌种1]和[具体菌种2]。通过分子系统发育和生物地理学确认了[具体菌种3]的身份。此外,菌丝拮抗试验证实了物种差异,菌株还通过形态学和蛋白质谱进一步区分。在液体培养和固态发酵中,[具体菌种3]的生物量和多糖产量与[具体菌种1]和[具体菌种2]明显不同。[具体菌种3]在第9天的深层培养中获得了最高多糖产量(4.52±0.83 g/L)。[具体菌种3]在木质纤维素固体底物中也实现了最高的线性生长。因此,通过在聚丙烯袋中进行固态发酵成功获得了担子果,而在人工固体底物中未产生[具体菌种1]和[具体菌种2]的蘑菇。[具体菌种3]是一种在美国经常采集到的菌种,显示出是一种有前途的用于生产膳食补充剂的多糖生产者。

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