Department of Biotechnology, Punjabi University Patiala, Punjabi, India.
Botany and Microbiology Department, Faculty of Science, King Saud University, Riyadh, Saudi Arabia.
PLoS One. 2021 Mar 25;16(3):e0248116. doi: 10.1371/journal.pone.0248116. eCollection 2021.
Freshwater lakes present an ecological border between humans and a variety of host organisms. The present study was designed to evaluate the microbiota composition and distribution in Dal Lake at Srinagar, India. The non-chimeric sequence reads were classified taxonomically into 49 phyla, 114 classes, 185 orders, 244 families and 384 genera. Proteobacteria was found to be the most abundant bacterial phylum in all the four samples. The highest number of observed species was found to be 3097 in sample taken from least populated area during summer (LPS) whereas the summer sample from highly populated area (HPS) was found most diverse among all as indicated by taxonomic diversity analysis. The QIIME output files were used for PICRUSt analysis to assign functional attributes. The samples exhibited a significant difference in their microbial community composition and structure. Comparative analysis of functional pathways indicated that the anthropogenic activities in populated areas and higher summer temperature, both decrease functional potential of the Lake microbiota. This is probably the first study to demonstrate the comparative taxonomic diversity and functional composition of an urban freshwater lake amid its highly populated and least populated areas during two extreme seasons (winter and summer).
淡水湖泊是人类与各种宿主生物之间的生态边界。本研究旨在评估印度斯利那加达尔湖的微生物群落组成和分布。非嵌合序列读取被分类为 49 个门、114 个纲、185 个目、244 个科和 384 个属。在所有四个样本中，变形菌门被发现是最丰富的细菌门。在夏季人口最少的地区（LPS）采集的样本中，观察到的物种数量最多，为 3097 种，而在夏季人口最多的地区（HPS）采集的样本在分类多样性分析中被发现是所有样本中最多样化的。使用 QIIME 输出文件进行 PICRUSt 分析以分配功能属性。这些样本表现出微生物群落组成和结构的显著差异。功能途径的比较分析表明，人口密集地区的人为活动和较高的夏季温度都降低了湖泊微生物群的功能潜力。这可能是首次在两个极端季节（冬季和夏季）在人口密集和人口最少的地区之间研究城市淡水湖的比较分类多样性和功能组成。
