College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing 210095, P.R. China.
Zibo Service Center for Animal Husbandry and Fishery, Zibo 255000, Shandong Province, P.R. China.
J Anim Sci. 2021 May 1;99(5). doi: 10.1093/jas/skab089.
Oxidative stress seriously affects poultry production. Nutritional manipulations have been effectively used to alleviate the negative effects caused by oxidative stress. This study investigated the attenuating effects and potential mechanisms of dietary taurine on the growth performance and meat quality of broiler chickens challenged with hydrogen peroxide (H2O2). Briefly, a total of 192 male Arbor Acres broilers (28 d old) were randomly categorized into three groups: non-injection of birds on basal diets (control), 10.0% H2O2 injection of birds on basal diets (H2O2), and 10.0% H2O2 injection of birds on basal diets supplemented with 5 g/kg taurine (H2O2 + taurine). Each group consisted of eight cages of eight birds per cage. Results indicated that H2O2 administration significantly reduced growth performance and impaired breast meat quality by decreasing ultimate pH and increasing shear force value (P < 0.05). Dietary taurine improved the body weight gain and feed intake and decreased feed/gain ratio of H2O2-challenged broilers. Meanwhile, oxidative stress induced by intraperitoneal injection of H2O2 suppressed the nuclear factor-κB (NF-κB) signaling and initiated autophagy and apoptosis. Compared with the H2O2 group, taurine supplementation restored the redox status in the breast muscle by decreasing levels of reactive oxygen species and contents of oxidative products and increasing antioxidant capacity (P < 0.05). Moreover, upregulated mRNA expression of NF-κB signaling-related genes, including NF-κB subunit 1 (p50) and B-cell CLL/lymphoma 2 (Bcl-2), and enhanced protein expression of NF-κB were observed in the H2O2 + taurine group (P < 0.05). Additionally, dietary taurine decreased the expression of caspase family, beclin1, and microtubule-associated protein 1light chain 3 beta (LC3-II; P < 0.05), thereby rescuing autophagy and apoptosis in breast muscle induced by H2O2. Collectively, dietary supplementation with taurine effectively improves growth performance and breast meat quality of broilers challenged with H2O2, possibly by protecting against oxidative injury and modulating cell death signaling.
氧化应激严重影响家禽生产。营养调控已被有效用于减轻氧化应激引起的负面影响。本研究探讨了饲粮牛磺酸对过氧化氢（H2O2）应激肉鸡生长性能和肉品质的缓解作用及其潜在机制。简而言之，将 192 只 28 日龄雄性爱拔益加肉鸡（Arbor Acres）随机分为 3 组：基础日粮不注射组（对照组）、基础日粮 10.0%H2O2 注射组（H2O2 组）和基础日粮 10.0%H2O2 注射添加 5 g/kg 牛磺酸组（H2O2+牛磺酸组）。每组 8 个重复，每个重复 8 只鸡。结果表明，H2O2 处理显著降低了肉鸡的生长性能，通过降低最终 pH 值和增加剪切力值来损害胸肉品质（P<0.05）。饲粮牛磺酸提高了 H2O2 应激肉鸡的体重增重和采食量，降低了料重比。同时，H2O2 腹腔注射诱导的氧化应激抑制了核因子-κB（NF-κB）信号通路，并引发自噬和细胞凋亡。与 H2O2 组相比，牛磺酸补充剂通过降低活性氧和氧化产物含量以及提高抗氧化能力来恢复胸肌的氧化还原状态（P<0.05）。此外，在 H2O2+牛磺酸组中，NF-κB 信号通路相关基因 NF-κB 亚基 1（p50）和 B 细胞 CLL/淋巴瘤 2（Bcl-2）的 mRNA 表达上调，NF-κB 蛋白表达增强（P<0.05）。此外，饲粮牛磺酸降低了半胱天冬酶家族、beclin1 和微管相关蛋白 1 轻链 3β（LC3-II；P<0.05）的表达，从而挽救了 H2O2 诱导的胸肌自噬和细胞凋亡。综上所述，饲粮补充牛磺酸可有效改善 H2O2 应激肉鸡的生长性能和胸肉品质，可能通过防止氧化损伤和调节细胞死亡信号来实现。
